Inner hair cell ribbon synapse plasticity might be molecular basis of temporary hearing threshold shifts in mice.

Recent studies have reported that noise exposure at relatively low intensities can cause temporary threshold shifts (TTS) in hearing. However, the mechanism underlying the TTS is still on debate. Here, we report that an acoustic stimulation (100 dB SPL, white noise) induced TTS in mice, with the maximal ABR threshold elevations seen on the 4(th) day after noise exposure.

Effect evaluation of repeated debridement after endoscopic sinus surgery.

Objective: To investigate the effect of early phase debridement by the different intervention frequencies on postoperative symptoms recovery and turnover of mucosa after functional endoscopic sinus surgery (FESS).

Methods: 67 patients undergone FESS were divided into intervention group and control group. Intranasal corticosteroids, macrolides antibiotics and postoperative saline douching were used in both groups.

[Culture, identification and label of embryonic rat neural stem cells].

Objective: To explore the methods of culture, identification and label of embryonic rat neural stem cells.

Method: The cells isolated from fetal rat hippocampus were identified with nestin immunocytochemical fluorescent staining. The cellular multiplication was observed by immunocytochemical fluorescence co-label after accession of BrDU.

[Suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA].

Objective: To study the suppression of proliferation of Hep-2 cells by stable expression of anti-sense perlecan cDNA.

Method: In this study, the plasmids of recombination eukaryotic expression vector perlecan anti-sense cDNA (pAP) were transfected into Hep-2 cells by using cationic liposome (lipofectamine 2000) and divided into three groups: non-transfected group, WT group; transfection with no load carrier, neo group; and transfection with the pAP plasmid, pAP group. Semi quantify RT-PCR, western blot assay and MTT assay were used to detected the expression of perlecan mRNA and protein in the three groups; the level of cell proliferation; and the responsivity of basic fibroblast growth factor (bFGF).

[Neurofibromatosis type 2].

Objective: To recognise the predisposing factors, clinical manifestations, diagnosis and treatment of neurofibromatosis type 2 (NF2).

Method: The clinical data of one NF2 case was reported and the literatures were also reviewed.

Result: The patient was diagnosed at a much later stage than onset.

[Excitotoxic effects of glutamate on the in vitro spiral ganglion cells].

Objective: To explore the excitotoxic effects of glutamate on the in vitro primarily cultured spiral ganglion cell (SGC) of rat.

Method: Spiral ganglion cells (SGCs) were cultured in vitro for 4 days, and exposed to 1 mmol/L glutamate for 24 hours. Damaged cells double-labeling with Hoechest33258 and PI were observed by fluorescence microscope.

[Application of rat tail collagen in the primary culture of rats marginal cells of stria vascularis].

Objective: To study the feasibility of application of rat-tail collagen in the primary culture of rats marginal cells of stria vascularis.

Method: The effect of self-made rat-tail collagen on the primary culture of rats marginal cells of stria vascularis was observed and estimated.

Result: When cochlear stria vascularis fragment was isolated and cultured for 24 h, a few culture cells appeared around the fragments.

[Experimental study of primary culture methods for spiral ganglion cells of newborn rats].

Objective: To optimize culture condition of spiral ganglion cells (SGCs) in vitro and to obtain highly purified SGCs.

Method: The spiral ganglions from newborn rats were digested in 0.25% trypsin and 0.

[The effects of sodium salicylate on the expression of GABAalpha, NR1 and hearing response properties of inferior colliculus neurons in mice].

Aim: To study the effects of sodium salicylate on the expression of GABAalpha NR1 and hearing response properties of inferior colliculus neurons in mice.

Methods: Thirty-six kunming mice were divided into three groups (A, B, C,). The expression of GABAalpha NR1 were measured by using RT-PCR.

[Expression of perlecan in laryngeal carcinoma cell and its significance].

Objective: To study the expression of perlecan in human laryngeal carcinoma cells and its significance.

Method: The expression of perlecan mRNA in human laryngeal carcinoma cells, Hep-2 cells were investigated by using the techniques of semi-quantify RT-PCR. The expression of perlecan in the Hep-2 cells was investigated by using the techniques of immunohistochemistry.

[Expression of vascular endothelial growth factor and its receptors in laryngeal carcinoma cell and its significance].

Objective: To study the expression of vascular endothelial growth factor (VEGF) and both the Flt-1 and KDR high affinity VEGF receptors in human laryngeal carcinoma cells and its significance.

Method: In this study, we investigated the expression of VEGF mRNA and both the Fit-1 mRNA and KDR mRNA high affinity VEGF receptors in human laryngeal carcinoma cells using techniques of semi-quantify RT-PCR.

Result: It was showed that the expression level of VEGF mRNA was significantly increased in human laryngeal carcinoma cells as compared with the normal cells, Hacat cells (P <0.

[Construction of recombinant adenovirus containing human Bcl-2 and its expression in the spiral ganglion cells].

Objective: To construct the adenoviral vector containing human Bcl-2 gene and to study the expression of the gene in the spiral ganglion cells (SGC) in vitro.

Methods: Human Bcl-2 cDNA obtained from the plasmid pUC-CAGGS/Bcl-2 was cloned into the plasmid pAdTrack-CMV. Then, pAdTrack/Bcl-2 was cotransferred with adenoviral backbone vector into E.

Effects of neuroglobin gene transfer in vivo on hearing response properties of neurons in the inferior colliculus in mice after administration of sodium salicylate.

The effects of neuroglobin (NGB) gene transfer in vivo mediated by GeneJamer on the hearing response properties of the inferior colliculus (IC) neurons in mice after administration of sodium salicylate were studied. Forty-eight Kunming mice were divided into 4 groups (n=12 in each group): Group A1 (negative control);Group A2 (positive control);Group B, sodium salicylate (450 mg/kg every day) + pEGFP-C1;Group C, sodium salicylate (450 mg/kg every day) + pEGFP-NGB. The GeneJamer and pEGFP-NGB were mixed and injected into IC neurons in mice.