Serum Pharmacology Combining Network Pharmacology to Discover the Mechanism of Total Flavonoids in Aronia Melanocarpa Fruit for Acute Lung Injury.

This comprehensive evaluation evaluates the activity of total flavonoids from Atractylodes macrocephala (AMF) in treating acute lung injury (ALI) and explores the mechanism of action of AMF total flavonoids in treating ALI. Method: UV spectrophotometry was used to determine the in vitro antioxidant activity of total flavonoids in AMF; Establishment of ALI model mice induced by lipopolysaccharide (LPS); Analysis of total flavonoid composition and blood migration components in AMF based on UHPLC-MS/MS technology; Analyze the active compounds, targets, and mechanisms of action of AMF total flavonoids in treating ALI through serum drug chemistry combined with network pharmacology. The results of in vitro antioxidant combined with in vivo anti-inflammatory experiments showed that AMF total flavonoids exhibited excellent therapeutic effects on ALI.

Metabolomics analysis reveals the effect of fermentation on the chemical composition and antioxidant activity of Root.

Fermentation is an effective means of enhancing the nutritional value of natural medicines, however, it is unclear how the metabolites changed during the fermentation of root (PLR). This study intends to elucidate how the active constituents and antioxidant activity of PLR change during fermentation. The study examined the levels of total glucosides of paeony (TGP), total flavonoids content (TFC), total phenols content (TPC), and antioxidant capability by high performance liquid chromatography (HPLC) and spectrophotometry.