A Review on Investigating the Interactions between Nanoparticles and the Pulmonary Surfactant Monolayer with Coarse-Grained Molecular Dynamics Method.

Pulmonary drug delivery has garnered significant attention due to its targeted local lung action, minimal toxic side effects, and high drug utilization. However, the physicochemical properties of inhaled nanoparticles (NPs) used as drug carriers can influence their interactions with the pulmonary surfactant (PS) monolayer, potentially altering the fate of the NPs and impairing the biophysical function of the PS monolayer. Thus, the objective of this review is to summarize how the physicochemical properties of NPs affect their interactions with the PS monolayer.

Numerical investigations of translocation characteristics of nano-silica lunar dust across pulmonary surfactant monolayer.

The interactions between nano-silica lunar dust (NSLD) on the moon surface and pulmonary surfactant (PS) monolayer will pose risks to astronaut health in future manned lunar exploration missions, but the specifics of these interactions are unknown. This study investigates them using the coarse-grained molecular dynamics method considering different sizes (5, 10, and 15 nm) and shapes (sphere, ellipsoid, and cube), with special focus on the unique morphology of NSLDs with bugles. The key findings are as follows: (1) The 10 nm and 15 nm NSLDs embed in the PS monolayer through the major sphere of spherical-type, major ellipsoid of ellipsoidal-type, or one edge of cubic-type NSLDs upon contact the PS monolayer.

Exon 9 Expression Is a Potential Prognostic Marker of Unfavorable Recurrence-Free Survival in Patients with Tongue Squamous Cell Carcinoma.

This study tried to assess the prognostic value of semaphorin (SEMA) family genes in patients with tongue squamous cell carcinoma (TSCC) and the potential epigenetic alterations of the genes. The part of third-level TSCC data in The Cancer Genome Atlas-Head and Neck Squamous Cell Carcinoma (TCGA-HNSC) was extracted using the UCSC Xena browser for analysis. Among 20 SEMA genes examined, 7 were markedly upregulated, while 8 were substantially decreased in TSCC tissues compared with adjacent normal tissues.

Substance P participates in periodontitis by upregulating HIF-1α and RANKL/OPG ratio.

Background: Both substance P and hypoxia-inducible factor 1 alpha (HIF-1α) are involved in inflammation and angiogenesis. However, the relationship between substance P and HIF-1α in rat periodontitis is still unknown.

Methods: Ligation-induced rat periodontitis was established to observe the distribution and expression of substance P and HIF-1α by immunohistochemistry.

The effects of Sema3A overexpression on the proliferation and differentiation of rat gingival mesenchymal stem cells in the LPS-induced inflammatory environment.

Semaphorin3A has been identified as a potent osteoprotective factor that simultaneously inhibits bone resorption and promotes bone formation. The present study demonstrates the effect of the overexpression of Sema3A on the proliferation and differentiation of rat gingival mesenchymal stem cells (GMSCs) in the lipopolysaccharide (LPS)-induced inflammatory environment. rGMSCs were transfected with viral stocks of pLenO-GTP-Sema3A (Lv-Sema3A group) or pLenO-GTP (Lv-NC group), with rGMSCs as a control.

suppresses the progression of oral squamous cell carcinoma through wnt/β-catenin pathway by targeting phospholipase D1.

The current study aimed to explore the function of on the progression of oral squamous cell carcinoma (OSCC) and relevant molecular mechanisms. Expression profile of in OSCC tissues and cells was detected using quantitative real-time polymerase chain reaction (qRT-PCR) method. Chi-square test was performed to estimate the relationship between and clinical parameters of OSCC cases.

Semaphorin 3A gets involved in the establishment of mouse tooth eruptive pathway.

The accurately establishment of the eruptive pathway is of vital importance. The mechanisms governing tooth eruption pathway remain little known. This study is to elucidate the roles of Semaphorin 3A (Sema 3A) in mouse tooth eruptive pathway.

promotes cell proliferation, migration, and invasion through activating PI3K/AKT signaling pathway in oral squamous cell carcinoma.

This research aimed to explore the function of protease activated receptor 2 () in oral squamous cell carcinoma (OSCC) development and progression, as well as underlying molecular mechanism. Tissue samples were collected from 115 OSCC patients. Quantitative real-time PCR (qRT-PCR) was performed to measure the expression of mRNA in OSCC tissues and cells.

[Effect of hypoxia on the expression of HIF-1alpha and VEGF in human periodontal ligament cells in vitro].

Purpose: To investigate the effect of hypoxia on the expression of HIF-1alpha and VEGF in human periodontal ligament cells (hPDLCs) in vitro.

Methods: HPDLCs were cultured in Dulbecco's modified Eagle's medium (DMEM) and subcultured at confluence. Experiments were carried out with the third passage of hPDLCs populations.

[Effect of epidermal growth factor on transcription of MCP-1 in rat dental follicle cells].

Purpose: To study the effects of epidermal growth factor (EGF) on the secretion of monocyte chemotactic protein-one (MCP-1) in rat dental follicle cells.

Methods: Dental follicle was harvested from 4-6 days postnatal Wistar rats and cultured in alpha-MEM with 15% FBS. Cells in the third passage were incubated with EGF at different concentrations to choose the best dosage.

[Proliferation, apoptosis and expression of tissue non-specific alkaline phosphatase in cells of stratum intermedium during amelogenesis in postnatal stage of BALB/c mouse].

Purpose: To study the apoptosis and expression of proliferating cell nuclear antigen(PCNA),tissue non-specific alkaline phosphatase(TNSALP) of stratum intermedium cell in postnatal stage of BALB/c mouse.

Methods: Fifty six BALB/c mice in different developmental days were sacrificed and their bilateral mandibular first molar germs with surrounding alveolar bone were taken out, then the tissues were fixed with 4% paraformaldehyde at 4degrees centigrade overnight, dehydrated, embedded in paraffin and serially sectioned at 5mum.Tdt-mediated Dutp nick end labeling (TUNEL) and immunohistochemical assay were adopted to determine the tissue distribution and cellular localization of bcl-2, PCNA and TNSALP,respectively.