Publications by authors named "Kaili Duan"
Objectives: It has been reported that serum Clara cell secreted protein 16 (CC16) is a potential biomarker for lung injury diseases, but currently, there is no other method that is faster, more accurate, or more sensitive being applied in clinical practice apart from ELISA. The current study was designed to established a magnetic nanoparticles chemiluminescence immunoassay (MNPs-CLIA) for highly sensitive automated detection of serum Clara cell secretory protein 16 (CC16), and validated its diagnostic performance for lung disease.
Methods: The study included the expression of CC16 recombinant protein, the preparation and screening of its monoclonal antibody (MAb), as well as the construction, optimization and analytical evaluation of the MNPs-CLIA method.
Article Synopsis
- * The study identifies the ING protein Fng2 in wheat head blight fungus, revealing that its deletion leads to defects in growth, reproduction, and other critical functions, impacting gene expression significantly.
- * Fng2 negatively regulates histone H4 acetylation and interacts with a histone deacetylase (FgRpd3) to control the expression of thousands of genes, which is essential for fungal development and pathogenicity.
A non-pheromone GPCR is essential for meiosis and ascosporogenesis in the wheat scab fungus.
Proc Natl Acad Sci U S A
October 2023
Article Synopsis
- Meiosis is crucial for genetic diversity in filamentous fungi, but its regulation remains unclear, particularly in dikaryotic and diploid cells that depend on internal environments rather than pheromones.
- The study identifies Gia1, a non-pheromone G protein-coupled receptor (GPCR), as essential for ascosporogenesis, with a mutant showing normal fruiting body development but inability to undergo meiosis, which can be partially rescued by activating the Gpmk1 pathway.
- Compared to its paralog, Gia2, Gia1 has distinct expression profiles and signaling mechanisms, highlighting its role in regulating sexual reproduction processes in filamentous ascomycetes.
Plant hormones are important for regulating growth, development, and plant-pathogen interactions. Some of them are inhibitory to growth of fungal pathogens but the underlying mechanism is not clear. In this study, we found that hyphal growth of Fusarium graminearum was significantly reduced by high concentrations of IAA and its metabolically stable analogue 2,4-dichlorophenoxyacetic acid (2,4-D).
View Article and Find Full Text PDFEffect of Dibutyl Phthalate on the Tolerance and Lipid Accumulation in the Green Microalgae Chlorella vulgaris.
Bull Environ Contam Toxicol
September 2018
Article Synopsis
- Researchers cultured Chlorella vulgaris with varying concentrations of the plasticizer dibutyl phthalate (DBP) over 10 days to study its effects on cell density and lipid production.
- High DBP concentrations (50 and 100 mg/L) negatively impacted the cell density and lipid content of C. vulgaris, while low concentrations (5, 10, and 20 mg/L) enhanced growth and lipid accumulation.
- The study found that C. vulgaris could effectively remove DBP from water, suggesting its potential use for bioremediation in contaminated environments.