Epidemiological and genetic characteristics of EV71 in hand, foot, and mouth disease in Guangxi, southern China, from 2010 to 2015.

Hand, foot, and mouth disease (HFMD) is a significant public health challenge in China. Human enterovirus 71 (EV71) is regarded as the predominant causative pathogen of HFMD. Since 2015, two inactivated EV71 vaccines have been approved in mainland China, and because their use could change the HFMD pathogen spectrum, this should now be monitored.

[Survey and anslysis of infection rate of dog rabies in the regions with high incidence of human rabies].

Objective: To investigate the situation of dog rabies and analyze it's relationship with human rabies.

Methods: In Guizhou, Guangxi and Hunan provinces which suffered from rabies most heavily, one or two cities were selected respectively from regions with high-, middle-, low-incidence rate of human rabies as investigation spots where Dogs' brain specimens were collected and detected with both direct immunofluorescence assay (IFA) and RT-PCR.

Results: A total of 2887 specimens were collected and 66 of these were positive for IFA and RT-PCR.

[A research on rotaviral diarrhea outbreak in Guangxi Province].

Objective: To analyze epidemiological characters of an outbreak of rotavirus diarrhea in Daxing County, Guangxi Province.

Methods: Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR.

[Study on the variations of hepatitis B virus status among people having received hepatitis B vaccine].

Objective: To study the epidemiological pattern and trends of hepatitis B virus (HBV) in the area where people had been immunized by HBV vaccine for long time.

Methods: Through cluster sampling and cross-sectional study, relative information and blood samples from people in Long-an county by families were collected. Signals of HBV infection were tested by solid-phase reverse immunosorbent test.

[Variations of hepatitis B virus infection epidemic pattern after long-term HBV vaccine immunization].

Objective: To study the epidemic pattern and trend of HBV infection in the area where the people had been immunized by HBV vaccine for 20 years.

Methods: The whole sampling method was applied in combination with cross-sectional investigation. Blood samples were taken from every member of families.

[The immuno-protect study of a hepatitis E virus ORF2 peptide expressed in E. coli].

A hepatitis E virus (HEV) ORF2 peptide expressed in an unfused pattern in E. coli, NE2, can self-assembly into homodimers and oligomers, and the immuno-reactivity of the dimers or oligomers to HEV infected serum is much stronger than monomer, which suggested that some important conformational epitopes be better exposed in dimer or oligomer form. Three Rhesus monkeys were vaccinated with three doses (10 microg/dose) purified NE2 in Freud's adjuvant under a schedule of Od, 10d, and 30d.