Four ClEF1A genes involved in self-incompatibility in 'Xiangshui Lemon' confer early fowering and increase stress tolerance in transgenic Arabidopsis.

The plant elongation factor eEF1A is involved in coregulating not only the translation of proteins and controlling translation-related signaling but also in signaling associated with cell growth, stress response and motility, controlling apoptosis and responding to adversity in plants. In this study, four eEF1A genes, namely, ClEF1A-1, ClEF1A-2, ClEF1A-3 and ClEF1A-4, were identified from the genomic and ubiquitin-modified omics data of the 'Xiangshui Lemon', and bioinformatics analysis revealed that these four genes have relatively similar structures with conserved sequences; ClEF1A-1 and ClEF1A-4 were highly expressed in pollen, and temporal expression analysis demonstrated that the expression of ClEF1As was significantly greater under self-pollination than under cross-pollination. All four genes were localized in the nucleus.

Overexpression of MiSPL3a and MiSPL3b confers early flowering and stress tolerance in Arabidopsis thaliana.

SQUAMOSA promoter-binding protein-like (SPL) family genes play an important role in regulating plant flowering and resistance to stress. However, understanding the function of the SPL family in mango is still limited. In a previous study, two MiSPL3 genes, MiSPL3a and MiSPL3b (MiSPL3a/b), were identified in 'SiJiMi' mango and exhibited the highest expression in flowers at the initial flowering stage [24].

Functional studies of four MiFPF genes in mango revealed their function in promoting flowering in transgenic Arabidopsis.

Flowering promoting factor (FPF) genes play a substantial regulatory role in the process of flowering. In the present study, four MiFPF genes, namely, MiFPF1, MiFPF2, MiFPF3a, and MiFPF3b, were obtained from mango (Mangifera indica L.).