Development of a magnetic separation method for rapid isolation and enrichment of bacteria in raw pork using chitosan functionalized magnetic FeO@MIL-100(Fe) composites.

In this study, a pretreatment method based on a magnetic capture probe for the rapid isolation and enrichment of bacteria from raw pork was developed. The chitosan immobilized FeO@MIL-100(Fe) was prepared as a capture probe for total bacterial counts through the electrostatic interaction of positively charged chitosan and the negatively charged substances on the surface of bacteria. The interference of matrix in pork samples on this method was studied and removed by differential centrifugation.

M13 phage as network frame for the quantification of Pb based on the Pb-induced in-situ growth of gold nanoparticles.

Routine detection of Pb is of great importance to environmental monitoring because of its deleterious effects on human health and environment. In this work, an easy-to-operate method for the one-step detection of Pb was developed based on the phage-Pb interactions and their influence on the surface plasmon resonance (SPR) absorption band of AuNPs. AuNPs grow in-situ on the surface of M13 phages and the resulting phage-AuNP networks were directly used for lead sensing.