Roles of endothelial cells in the regulation of cell motility via lysophosphatidic acid receptor-2 (LPA) and LPA in osteosarcoma cells.

Lysophosphatidic acid (LPA) signaling via LPA receptors (LPA to LPA) exhibits a variety of biological responses. In tumor microenvironment, endothelial cells promote cancer cell functions. In this study, we investigated the roles of endothelial cells in the regulation of cell motile activity via LPA and LPA in human osteosarcoma MG-63 cells.

Cooperation of G12/13 and Gi proteins via lysophosphatidic acid receptor-2 (LPA) signaling enhances cancer cell survival to cisplatin.

Lysophosphatidic acid (LPA) through six subtypes of G protein-coupled LPA receptors (LPA to LPA) mediates a variety of cancer cell functions. The aim of this study was to evaluate the cooperative effects of G12/13 and Gi proteins through LPA on cancer cell survival to cisplatin (CDDP). In cell survival assay, cells were treated with CDDP every 24 h for 2 days.

Different effects of lysophosphatidic acid receptor-2 (LPA) and LPA on the regulation of chemoresistance in colon cancer cells.

Lysophosphatidic acid (LPA) is a simple physiological lipid and exhibits several biological functions by binding to G-protein-coupled LPA receptors (LPA receptor-1 (LPA) to LPA). The present study aimed to evaluate whether LPA signaling LPA and LPA is involved in the chemoresistance to anticancer drugs in colon cancer DLD1 cells. In cell survival assay, cells were treated with fluorouracil (5-FU) every 24 h for 2 days.

Lysophosphatidic acid receptor-2 (LPA)-mediated signaling enhances chemoresistance in melanoma cells treated with anticancer drugs.

Lysophosphatidic acid (LPA) signaling through LPA receptors (LPA to LPA) regulates a variety of malignant properties in cancer cells. Recently, we show that LPA expression is elevated by long-term cisplatin (CDDP) treatment in melanoma A375 cells. In the present study, we investigated whether LPA-mediated signaling is involved in the modulation of chemoresistance in A375 cells.

Regulation of cell survival through free fatty acid receptor 1 (FFA1) and FFA4 induced by endothelial cells in osteosarcoma cells.

Free fatty acid receptor 1 (FFA1) and FFA4 belong to a family of free fatty acid (FFA) receptors. FFA1- and FFA4-mediated signaling regulates a variety of malignant properties in cancer cells. It is known that stromal cells in the tumor microenvironment promote tumor progression.

Effects of lysophosphatidic acid (LPA) receptor-2 (LPA) and LPA on the regulation of chemoresistance to anticancer drug in lung cancer cells.

Lysophosphatidic acid (LPA) mediates a variety of biological functions via the binding of G protein-coupled LPA receptors (LPA receptor-1 (LPA) to LPA). This study aimed to investigate the roles of LPA and LPA in the modulation of chemoresistance to anticancer drug in lung cancer A549 cells. In cell survival assay, cells were treated with cisplatin (CDDP) every 24 h for 2 days.

LPA-mediated signaling induced by endothelial cells and anticancer drug regulates cellular functions of osteosarcoma cells.

Lysophosphatidic acid (LPA) signaling via LPA receptors (LPA to LPA) regulates a variety of malignant properties of cancer cells. It is known that endothelial cells promote tumor progression and chemoresistance. The present study aimed to investigate the roles of LPA in cellular functions modulated by endothelial cells and anticancer drug in osteosarcoma cells.

Rapid establishment of highly migratory cells from cancer cells for investigating cellular functions.

Cell migration is closely involved in cancer cell invasion into surrounding tissue and metastasis to the distant organs. It is crucial for understanding the molecular mechanisms that regulate cell migration in cancer cells. The aim of this study is to establish a rapid induction method of highly migratory cells from cancer cells.

Modulation of chemoresistance by lysophosphatidic acid (LPA) signaling through LPA in melanoma cells treated with anticancer drugs.

Lysophosphatidic acid (LPA) signaling via LPA receptors (LPA to LPA) contributes to the promotion of malignant potency in cancer cells. The cell motile activity are stimulated through the induction of LPA in melanoma cells treated with anticancer drugs. The present study aimed to investigate whether LPA signaling via LPA regulates chemoresistance in melanoma A375 cells.

Promotion of cell-invasive activity through the induction of LPA receptor-1 in pancreatic cancer cells.

Lysophosphatidic acid (LPA) is a simple biological lipid and mediates several biological functions with LPA receptors (LPA to LPA). In the present study, to assess whether LPA receptors promote cell-invasive activity of pancreatic cancer cells, highly invasion PANC-R9 cells were established from PANC-1 cells, using Matrigel-coated Cell Culture Insert. The cell-invasive activity of PANC-R9 cells was shown to be approximately 15 times higher than that of PANC-1 cells.

Induction of GPR40 positively regulates cell motile and growth activities in breast cancer MCF-7 cells.

Free fatty acid (FFA) receptors belong to a member of G-protein-coupled receptors. GPCR 120 (GPR120) and GPR40 are identified as FFA receptors and activated via the binding of long- and medium-chain FFAs. The aim of this study was to assess the effects of GPR120 and GPR40 on cell motility and growth in breast cancer cells treated with tamoxifen (TAM).

Lysophosphatidic acid receptor-2 (LPA) and LPA regulate cellular functions during tumor progression in fibrosarcoma HT1080 cells.

Lysophosphatidic acid (LPA) receptors (LPA to LPA) regulate a variety of malignant properties in cancer cells. In the present study, we investigated the roles of LPA receptors in the promotion of cellular functions during tumor progression in fibrosarcoma cells. To obtain long-term anticancer drug treated cells, human fibrosarcoma HT1080 cells were treated with methotrexate (MTX) and cisplatin (CDDP) for 6 months.

Involvement of LPA signaling via LPA receptor-2 in the promotion of malignant properties in osteosarcoma cells.

Lysophosphatidic acid (LPA) signaling via G protein-coupled LPA receptors mediates various biological effects in cancer cells. This study aimed to investigate the roles of LPA receptors in the regulation of cellular functions during tumor progression in osteosarcoma cells. Long-term cisplatin (CDDP)-treated MG63-C and MG63-R7-C cells were generated from osteosarcoma MG-63 and highly-migratory MG63-R7 cells, respectively.

Involvement of FFA1 and FFA4 in the regulation of cellular functions during tumor progression in colon cancer cells.

Free fatty acid receptor 1 (FFA1) and FFA4 mediate a variety of biological responses through binding of medium- and long-chain free fatty acids. The aim of this study was to investigate an involvement of FFA1 and FFA4 in the regulation of cellular functions during tumor progression in colon cancer cells. The long-term fluorouracil (5-FU) and cisplatin (CDDP) treated cells were generated from DLD1 cells (DLD-5FU and DLD-CDDP cells, respectively).

Effects of LPA and LPA on the regulation of colony formation activity in colon cancer cells treated with anticancer drugs.

Lysophosphatidic acid (LPA) is a simple physiological lipid and exhibits a variety of cellular responses via the activation of G protein-coupled transmembrane LPA receptors (LPA receptor-1 (LPA) to LPA). The aim of our study was to investigate effects of LPA receptors on soft agar colony formation in colon cancer cells treated with anticancer drugs. DLD1 cells were treated with fluorouracil (5-FU) or cisplatin (CDDP) for at least six months (DLD-5FU and DLD-CDDP cells, respectively).

Involvement of LPA receptor-5 in the enhancement of cell motile activity by phorbol ester and anticancer drug treatments in melanoma A375 cells.

Lysophosphatidic acid (LPA) signaling through six subtypes of LPA receptors (LPA to LPA) regulates a variety of biological responses in cancer cells. The aim of our study was to evaluate an involvement of LPA receptors in the activation of cell motility by phorbol ester and anticancer drug treatments in melanoma A375 cells. Cells were treated with 12-O-tetradecanoylphorbol- 13-acetate (TPA) and phorbol-12,13-dibutyrate (PDBu) for 3 days.