Publications by authors named "Kai-yi Chen"

Mitochondrial G-quadruplexes are components that are potentially involved in regulating mitochondrial function and play crucial roles in the replication and transcription of mitochondrial genes. Consequently, it is imperative to develop probes that can detect mitochondrial G-quadruplexes to understand their functions and mechanisms. In this study, a triphenylamine fluorescent probe, TPPE, which has excellent cytocompatibility and does not affect the natural state of G-quadruplexes, was designed and demonstrated to localize primarily to the mitochondria.

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The FZP gene plays a critical role in the formation of lateral branches and spikelets in rice panicle architecture. This study investigates the qSBN7 allele, a hypomorphic variant of FZP, and its influence on panicle architectures in different genetic backgrounds. We evaluated two backcross inbred lines (BILs), BC5_TCS10 and BC3_TCS10, each possessing the homozygous qSBN7 allele but demonstrating differing degrees of spikelet degeneration.

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Vanilla () is a precious natural flavoring that is commonly used throughout the world. In the past, all vanilla used in Taiwan was imported; however, recent breakthroughs in cultivation and processing technology have allowed Taiwan to produce its own supply of vanilla. In this study, headspace solid-phase microextraction (HS-SPME) combined with GC-FID and GC-MS was used to analyze the volatile components of vanilla from different origins produced in Taiwan under different cultivation and processing conditions.

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Background: Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens.

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Article Synopsis
  • Global climate change is negatively impacting crop yields by exposing plants to a wider range of temperatures throughout their life cycles.
  • The study identifies 22 Quantitative Trait Loci (QTL) related to reproductive traits affected by temperature, revealing how different heat conditions influence traits like flower and fruit numbers.
  • The research provides a catalog of specific QTLs and potential breeding materials, which can help develop crop varieties that are more resilient to increasing temperatures in the future.
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Secondary branch number per panicle plays a crucial role in regulating grain number and yield in rice. Here, we report the positional cloning and functional characterization for (), a quantitative trait locus affecting secondary branch per panicle and grain number. Our research revealed that the causative variants of are located in the distal promoter region of (), a gene previously associated with the repression of axillary meristem development in rice spikelets.

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To mine new favorable alleles for tomato breeding, we investigated the feasibility of utilizing as a diverse panel of genome-wide association study through the restriction site-associated DNA sequencing technique. Previous attempts to conduct genome-wide association studies using were impeded by an inability to correct for population stratification and by lack of high-density markers to address the issue of rapid linkage disequilibrium decay. In the current study, a set of 24,330 SNPs was identified using 99 accessions from the Tomato Genetic Resource Center.

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The Orchidaceae is a diverse and ecologically important plant family. Approximately 69% of all orchid species are epiphytes, which provide diverse microhabitats for many small animals and fungi in the canopy of tropical rainforests. Moreover, many orchids are of economic importance as food flavourings or ornamental plants.

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Secondary branch number (SBN) is an important component affecting spikelet number per panicle (SPP) and yield in rice. During recurrent backcross breeding, four BCF populations derived from the high-yield donor parent IR65598-112-2 and the recurrent parent Tainan 13 (a local cultivar) showed discontinuous variations of SPP and SBN within populations. Genetic analysis of 92 BCF individuals suggested that both SPP and SBN are controlled by a single recessive allele.

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Tomato late blight caused by the oomycete pathogen Phytophthora infestans (Mont.) de Bary is a major threat to tomato production in cool and wet environments. Intensified outbreaks of late blight have been observed globally from the 1980s, and are associated with migration of new and more aggressive populations of P.

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Stunted lemma palea 1 (slp1) is a rice mutant that displays dwarfism, shortened inflorescence lengths, severely degenerated lemmas/paleas, and sterility. The SLP1 locus was mapped between markers RM447 and D275 in the distal region of the long arm of chromosome 8, using the F2 progeny derived from the cross between the Slp1/slp1 mutant (Oryza sativa subsp. japonica) and the variety Taichung Native 1 (TN1, O.

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The sweet potato sporamin promoter was used to control the expression in transgenic potato of the E. coli appA gene, which encodes a bifunctional enzyme exhibiting both acid phosphatase and phytase activities. The sporamin promoter was highly active in leaves, stems and different size tubers of transgenic potato, with levels of phytase expression ranging from 3.

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We report the cloning of Style2.1, the major quantitative trait locus responsible for a key floral attribute (style length) associated with the evolution of self-pollination in cultivated tomatoes. The gene encodes a putative transcription factor that regulates cell elongation in developing styles.

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The degree to which stigmas are exserted above the stamen in flowers is a key determinant of cross-pollination (and hence allogamy) in many plant species. Most species in the genus Lycopersicon are obligate or facultative outcrossers and bear flowers with highly exserted stigmas. In contrast, the cultivated tomato (Lycopersicon esculentum) bears flowers with flush or inserted stigmas promoting self-fertilization.

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We derive a formula for the distribution of the length T of the recombination interval containing a target gene and using N gametes in a region where R kilobases correspond to 1 cM. The formula can be used to calculate the number of meiotic events required to narrow a target gene down to a specific interval size and hence should be useful for planning positional cloning experiments. The predictions of this formula agree well with the results from a number of published experiments in Arabidopsis.

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