[Dynamics of Proliferation and Differentiation after Transplantation of Hematopoietic Cells in Mouse].

Objective: To investigate the effects of Listeria monocytogenes infection on hematopoietic stem and progenitor cell (HSPC) composition, cell cycle and cell colony-forming ability in mouse bone marrow.

Methods: The C57BL/6J mice were divided into infected group and control group. The mice in injected group were infected intraperitoneally with 6.

Decrease of galectin-3 in keratinocytes: A potential diagnostic marker and a critical contributor to the pathogenesis of psoriasis.

Psoriasis-specific proteins dysregulated in keratinocytes and involved in the pathophysiological process of psoriasis remains elusive. We report here that epidermal galectin-3 expression is significantly downregulated in lesional skin, but not in non-lesional skin in psoriasis patients, nor in a group of diseases known as psoriasiform dermatitis clinically and histologically similar to psoriasis. The deficiency of epidermal galectin-3 is sufficient to promote development of psoriatic lesions, as evidenced by more severe skin inflammation in galectin-3 knockout (gal3) mice, compared to wild-type mice, after imiquimod treatment, and in skin from gal3 mice grafted onto wildtype mice.

Association of anti-acidic ribosomal protein P0 and anti-galectin 3 antibodies with the development of skin lesions in systemic lupus erythematosus.

Objective: The specific autoantibodies and antigens that mediate systemic lupus erythematosus (SLE)-related organ injuries remain largely unknown. This study was undertaken to investigate the antibody-mediated immune response that leads to SLE skin lesions.

Methods: The study included 85 SLE patients with lupus-specific skin lesions and 31 without skin lesions.

Proteome analysis of Streptococcus suis serotype 2.

Outbreaks in humans, caused by Streptococcus suis serotype 2 (SS2), were reported in 1998 and 2005 in China. However, the mechanism of SS2-associated infection remains unclear. For the first time, a 2-D gel approach combined with MS was used to establish a comprehensive 2-D reference map for aiding our understanding of the pathogenicity of SS2.

Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T.

Aim: To screen the immunogenic membrane proteins of Shigella flexneri 2a 2457T.

Methods: The routine two-dimensional polyacrylamide gel electrophoresis (2-DE) and Western blotting were combined to screen immunogenic proteins of S. flexneri 2a 2457T.

Proteomic analysis and extensive protein identification from dry, germinating Arabidopsis seeds and young seedlings.

Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages.

[Investigation of constituents in siwu tang fractions by chromatographic and ESI-MS methods].

Objective: In order to discuss the chemical foundation of hematopoietic effect of Siwu Tang, three fractions of different polarities (C1, C2 and C3) were prepared from Siwu Tang and the characteristics of these fractions' constituents were investigated.

Method: Fraction C1, C2 and C3 of Siwu Tang and corresponding fractions of Siwu Tang's four ingredient drugs were analyzed and compared, synthetically using the three methods of high-performance thin layer chromatography (HPTLC), high-performance liquid chromatography (HPLC) and direct infusion electrospray ionization mass spectrometry (ESI-MS).

Result: Fraction C1 of Siwu Tang contained various types of compounds, including ferulic acid, paeoniflorin and supposedly ligustilide, etc.

[Application of PSD-MALDI-TOF-MS to protein identification].

Aim: To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.

Methods: The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.

Results: Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.

Proteomic analysis of ubiquitin-proteasome effects: insight into the function of eukaryotic initiation factor 5A.

The global effect of ubiquitin-proteasome (UP) inhibitors on leukemic cell proteome was analysed. A total of 39 protein spots, affected by UP inhibitors, were identified, including 11 new apoptosis-associated proteins. They are involved in different cellular functions and four were associated with caspase-3 activation.

[Application of bio-mass spectrometry in cellular signal transduction].

The quickly developing techniques of biological mass spectrometry (bio-MS) in recent years realized the high throughput identification of proteins by determining the accurate mass values of trypsin digested peptides and the randomly selected peptide sequence tags, and have been successfully used in the studies of protein interactions and post-translational modification such as the phosphorylation. Compared to the conventional approaches, the above techniques can identify all the phosphorylated proteins (including their phosphorylated amino acid sites) involved in a multi-signal pathway in a single experiment, and they have been developed into a hot-spot of proteomics. The three strategies for the application of bio-MS in the above fields are briefly reviewed.