Aquaporin 5 Plays a Role in Estrogen-Induced Ectopic Implantation of Endometrial Stromal Cells in Endometriosis.

Aquaporin 5 (AQP5) participates in the migration of endometrial cells. Elucidation of the molecular mechanisms associated with AQP5-mediated, migration of endometrial cells may contribute to a better understanding of endometriosis. Our objectives included identifying the estrogen-response element (ERE) in the promoter region of the AQP5 gene, and, investigating the effects of AQP5 on ectopic implantation of endometrial cells.

[AQP5 gene silencing inhibits proliferation and migration of ectopic endometrial glandular epithelial cells in endometriosis].

Objective: To investigate the effect of aquaporin 5(AQP5) on proliferation and migration of ectopic endometrial epithelial cells.

Methods: AQP5 shRNA interference fragments were designed and transfected into ectopic endometrial epithelial cells stably by lentivirus technology. Fluorescence quantitative RT-PCR and Western blotting were used to detect the AQP5 mRNA and protein expression, respectively.

Reduced expression of aquaporin 9 in tubal ectopic pregnancy.

Previous studies demonstrate significant roles for passive water channels (aquaporins, AQPs) in maintaining water homeostasis in cell membranes of endometrial cells during decidualisation and embryo implantation. However, there is little information regarding the role of AQPs in the human fallopian tube, specifically their role in human tubal ectopic pregnancy. In this study we took tissue samples from the site of implantation of tubal ectopic pregnancy (group 1, N = 30, mean age 32 years, range 23-42) and the corresponding non-implantation site in women undergoing salpingectomy for tubal pregnancy (group 2).

Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5.

Aquaporin (AQP)-dependent cell migration has broad implications in angiogenesis, tumor metastasis, wound healing, glial scarring and other events requiring cell movement. There are 13 isoforms of AQP (0-12) that have been identified in mammals. It is unclear whether AQP5 plays a role in the development of endometrial cancer.

[Effects of PRL-3 siRNA on the migration of endometriotic stromal cells].

Objective: To evaluate the effects of PRL-3 siRNA (small interfering RNA) on the migration of endometriotic stromal cells.

Methods: Primary endometriotic stromal cells were cultured in vitro. Then PRL-3 (phosphatase of regenerating liver-3) siRNA was transfected to silence the PRL-3 gene.

Phosphatase of regenerating liver-3: a novel and promising marker in human endometriosis.

Objective: To investigate the expression of phosphatase of regenerating liver-3 (PRL-3) in ectopic, eutopic, and normal endometria and explore its relationship with endometriosis.

Design: A clinical retrospective and molecular study.

Setting: Department of obstetrics, gynecology, and reproductive medicine.

Immunohistochemical detection of aquaporin expression in eutopic and ectopic endometria from women with endometriomas.

Objective: To investigate the expression of aquaporin (AQP) in eutopic and ectopic endometrial tissues from women with endometriomas.

Design: Controlled laboratory research.

Setting: Hospital-based unit for gynecology and obstetrics and research laboratories.

Plumbagin induces ROS-mediated apoptosis in human promyelocytic leukemia cells in vivo.

Plumbagin, a naphtoquinone from the roots of Plumbago zeylanica is known to possess anticancer and anti-bacterial activity. Based on the former finding of our group in vitro demonstrating its effectiveness in human promyelocytic leukemia cells, NB4, in this study we further revealed the mitochondrial pathway involved in plumbagin-induced apoptosis. We also found that the generation of ROS was a critical mediator in plumbagin-induced apoptosis, which would be abrogated completely by antioxidant, NAC.

Methotrexate therapy for cesarean section scar pregnancy with and without suction curettage.

Objective: To compare the clinical effects in women with cesarean scar pregnancy (CSP) who were treated with either methotrexate (MTX) regimen only or MTX regimen followed by dilation and curettage (D&C).

Design: Prospective consecutive clinical cohort study.

Setting: University hospital for obstetrics, gynecology, and reproductive medicine.

Hysteroscopic septum resection of complete septate uterus with cervical duplication, sparing the double cervix in patients with recurrent spontaneous abortions or infertility.

Objective: To evaluate the safety and efficacy of hysteroscopic septum resection of the complete septate uterus with cervical duplication in patients with recurrent spontaneous abortions or infertility.

Design: Prospective consecutive clinical study.

Setting: University hospital for obstetrics, gynecology, and reproductive medicine.

Locally elevated leukemia inhibitory factor in the inflamed fallopian tube resembles that found in tubal pregnancy.

Objective: To investigate the association between the expressions of leukemia inhibitory factor and the occurrence of tubal pregnancy.

Design: Prospective observational study.

Setting: University-based obstetrics and gynecology hospital.

Single large cystic adenomyoma of the uterus after cornual pregnancy and curettage.

A case of single large cystic adenomyoma of the uterus (anechoic area 1.6 cm in diameter) was diagnosed by surgery and histopathologic analysis more than 3 years after a transcervical curettage for an early right-cornual pregnancy.