[Optimization for ISSR-PCR system of traditional Chinese medicine Lysimachia christinae by orthogonal design].

In order to establish the stable andreliable ISSR-PCR System of Lysimachia christinae, L16 (4(5)) orthogonal design, which based on 7 levels of single factor experiment, were used in this study. The variance analysis was carried out by SPSS 19.0, and 5 main factors affecting the reaction system were optimized in 4 levels.

[Effect of different storage methods and time on seed germination of Bletilla striata].

Objective: Exploring seed storage method to offer a technological support for the seedling development of Bletilla striata.

Methods: Bletilla striata seeds were stored by means of sand storage, nature storage and low temperature storage for 120, 150 and 180 d respectively. The germination rates were measured with methods of seed germination on the Petri dish and difference of rates was compared by means of the SSR method.