Publications by authors named "Kai-Xin Fu"
Simple sequence repeat (SSR) markers are widely applied in studies of plant molecular genetics due to their abundance in the genome, codominant nature, and high repeatability. However, microsatellites are not always available for the species to be studied and their isolation could be time- and cost-consuming. To investigate transferability in cross-species applications, 102 primer pairs previously developed in ryegrass and tall fescue were amplified across three allogamous ryegrass species including Lolium rigidum, Lolium perenne and Lolium multiflorum.
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- Start codon targeted polymorphism (SCoT) analysis identified 37 whipgrass clones, revealing significant genetic diversity and population structure among them.
- The study generated 368 genetic fragments using 25 primer sets, with 77.21% of these fragments being polymorphic, indicating considerable variation among the clones.
- Results showed no correlation between genetic relationships and geographic origins, highlighting that most genetic diversity resides within geographic groups rather than between them, supporting SCoT as a useful method for genetic characterization.