Investigation of the effect of P14 promoter aberrant methylation on the biological function of human lung cancer cells.

Background: This study was conducted to investigate the effect of P14 promoter aberrant methylation on the biological function of human lung adenocarcinoma cells.

Methods: We used nested methylation-specific PCR (NMSP) to detect the methylation status of the p14ARF promoter region in SPCA1 and BEAS2B cell lines. The experimental groups were treated with 5-aza-2'-deoxycytidine (5-Aza).

The decreased expression of miR-625 predicts poor prognosis of esophageal squamous cell carcinoma.

Background: Previous study has detected the expression of miR-625 in esophageal squamous cell carcinoma (ESCC) and found that miR-625 was related to tumor depth, stage, and metastasis of ESCC. However, the prognostic value of miR-625 in ESCC has not yet been reported.

Methods: Real-time quantitative PCR was employed to measure the expression level of miR-625 in clinical ESCC tissues.