Effect of nortriptyline on Ca²⁺ handling in SIRC rabbit corneal epithelial cells.

To explore the effect of nortriptyline, a tricyclic antidepressant, on cytosolic free Ca²⁺ concentrations ([Ca²⁺]i) in corneal epithelial cells, [Ca²⁺]i levels in suspended SIRC rabbit corneal epithelial cells were measured by using fura-2 as a Ca²⁺-sensitive fluorescent dye. Nortriptyline at concentrations between 20-200 microM increased [Ca²⁺]i in a concentration-dependent manner. The Ca²⁺ signal was reduced partly by removing extracellular Ca²⁺.

Effect of the antidepressant sertraline on Ca2+ fluxes in Madin-Darby canine renal tubular cells.

The effect of the antidepressant sertraline on cytosolic-free Ca2+ concentrations ([Ca2+]i) in Madin Darby canine kidney (MDCK) cells is unclear. This study explored whether sertraline changed basal [Ca2+]i levels in suspended MDCK cells by using fura-2 as a Ca2+-sensitive fluorescent dye. Sertraline at concentrations between 1 and 100 microM increased [Ca2+]i in a concentration-dependent manner.