Effect of supplementation with CNCM I-1079 on vaccine response to an inactivated bacterial vaccine in young Japanese Black calves: A field trial.

group (SB group) calves were fed 2.0 × 10 CFU/day of in milk replacer after 2 wk of age. All calves received inactivated vaccine for , and at 3 wk of age and 3 wk later.

Preparation of Alkyl Di(-tolyl)sulfonium Salts and Their Application in Metal-Free C(sp)-C(sp) and C(sp)-C(sp) Bond Formations.

Alkyldiarylsulfonium salts were synthesized by a combination of active sulfonium species, prepared through the activation of diarylsulfoxide, and alkyl nucleophiles. The isolated sulfonium salts were subjected to the allylation and cyclopropanation of the active methylene compounds and metal-free C(sp)-C(sp) couplings via oxyallyl cation intermediates under mild conditions. The series of reactions included an umpolung strategy for the coupling of alkyl nucleophiles and metal-free C-C bond formation using sulfonium salts.

YO-001A, a new antifungal agent produced by Streptomyces sp. YO15-A001.

A new antifungal compound YO-001A was found from the culture broth of Streptomyces sp. YO15-A001, which was isolated from a soil sample collected in Toyama Prefecture. YO-001A was identified through morphological changes-based screening of the rice blast fungus, Pyricularia oryzae (P.

Phenotypic screening meets natural products in drug discovery.

The Nobel Prize in Physiology or Medicine 2015 was awarded for discoveries related to the control of parasitic diseases using natural products of microbial and plant origin. In current drug discovery programs, synthesized compounds are widely used as a screening source; however, this award reminds us of the importance of natural products. Here, we introduce our phenotypic screening methods based on changes in cell morphology and discuss their effectiveness and impact for natural products in drug discovery.

Changes in subcellular calcium transport in rat hearts during the calcium paradox.

Changes in the transport of calcium in the sarcoplasmic reticulum (SR) and mitochondria (MT), of the rat heart during calcium paradox were investigated. Calcium binding and uptake by SR in the paradox hearts were from about 1.5 to 2 times greater than in normal hearts, whereas in the MT of the paradox hearts they were about half those of normal hearts.

Toxicity of renal lysosomal contents on aortic smooth muscle cells in culture.

Effects of hog renal lysosomal contents and renin on cultured rat aortic smooth muscle cells (SMCs) were studied in attempts to determine mechanisms related to production of arterial lesions. The lysosomal contents rich in renin and with the pressor and the cytotoxic effects in vivo, inhibited the plating efficiency of SMCs in a dose- and incubation time-dependent manner. No inhibition occurred with the purified hog renin.

The production of small arterial lesions in nephrectomized rats by the administration of pure cathepsin D.

Pure cathepsin D was successfully obtained from rat liver by using affinity chromatography on pepstatin-aminohexyl-Sepharose gel. I.p.