Publications by authors named "Kai Ru"
Article Synopsis
- The Datong Basin served as a site for interaction and mixture between Han farmers from the Yellow River Valley and non-Han nomads from the Eastern Steppe, leading to uncertainty about the region's genetic history.
- Analysis of 289 mitogenomes showed high genetic diversity in the Datong population, with significant population expansion and close genetic ties to Northern Han subgroups, especially those from northern frontiers.
- The maternal gene pool of the Datong population was primarily composed of northern East Asian lineage (66.44%), with a smaller contribution from southern East Asians (31.49%) and negligible west Eurasian influence (2.07%), highlighting a stronger genetic connection to Yellow River farmers despite historical nomadic dominance
Backgrounds: Y-chromosomal haplotypes based on Y-short tandem repeats (STRs) and Y-single nucleotide polymorphisms/insertion and deletion polymorphisms (SNPs/InDels) are used to characterize paternal lineages of unknown male trace donors. However, Y-chromosomal genetic markers are not currently sufficient for precise individual identification. Microhaplotype (MH), generally < 200 bp on autosomes and consisting of two or more SNPs, was recently introduced in forensic genetics with the development of massive parallel sequencing technology and may facilitate identification and DNA mixture deconvolution.
View Article and Find Full Text PDFShort tandem repeats (STRs) are the preferred genetic markers in forensic DNA analysis, routinely measured by capillary electrophoresis (CE) method based on the fragment length features. While, the massive parallel sequencing (MPS) technology could simultaneously target a large number of intriguing forensic STRs, bypassing the intrinsic limitations of amplicon size separation and accessible fluorophores in CE, which is efficient and promising for enabling the identification of forensic biological evidence. Here, we developed a novel MPS-based Forensic Analysis System Multiplecues SetB Kit of 133-plex forensic STR markers (52 STRs and 81 Y-STRs) and one Y-InDel (M175) based on multiplex PCR and single-end 400 bp sequencing strategy.
View Article and Find Full Text PDFGuangdong province is situated in the south of China with a population size of 113.46 million. Hakka is officially recognized as a branch of Han Chinese, and She is the official minority group in mainland China.
View Article and Find Full Text PDFArticle Synopsis
- The research investigates the origins, diversification, and demographic history of the O1a-M119 lineage over the past 10,000 years, focusing on its impact on East and Southeast Asian populations, particularly the Han, Tai-Kadai, and Austronesian groups.
- The study utilized Y-chromosome sequences, including both new and previously collected data, to create a revised phylogenetic tree and analyze the geographic distribution of O1a-M119 sub-lineages among Chinese males.
- Findings reveal the continuous expansion of O1a-M119 and suggest a shared ancestry among the studied populations during the Neolithic Age, while highlighting the complexity of paternal genetic divergence across regions.
Article Synopsis
- More research is needed to understand the genetic diversity of Han populations across different regions in China, particularly in Henan province, which has historical significance as part of the ancient Huaxia.
- The study involved sequencing Y chromosomes from 60 males in Zhengzhou and revealed a high diversity of paternal lineages, indicating complex admixture over time.
- Findings suggest that the genetic diversity in modern Han populations is largely due to many ancient lineages from the Palaeolithic age, alongside a more recent expansion of a few predominant lineages.
[A pivotal role of cortactin, a CTTN encoding protein, in endocytosis of human colon cancer].
Zhonghua Yi Xue Za Zhi
February 2011
Objective: To explore the role of cortactin in endocytosis of colon cancer cells and clarify the significance of its over-expression in colon cancer tissues.
Methods: Immunohistochemistry and Western blot were employed to detect the expression of cortactin in benign and malignant tissues and cells. Cell endocytosis was examined in cancer cells after siRNA treatment and DNA transfection with plasmid encoding cortactin wild type and domain deletion mutants.