PDCD11 Stabilizes C-MYC Oncoprotein by Hindering C-MYC-SKP2 Negative Feedback Loop to Facilitate Progression of p53-Mutant Breast and Colon Malignancies.

C-MYC is a proto-oncoprotein whose dysregulation triggers tumorigenesis and tumor progression in ≈70% of cancer cases. It is presently demonstrated that aberrantly upregulated MYC is caused by the overexpressed and "extra-nucleolar" PDCD11 in p53-mutant breast and colon cancer cells, which is highly correlated to tumor progression, metastasis, and recurrence. In the nucleoplasm, PDCD11 binds to the TAD of C-MYC to prevent SKP2, a transcriptional target of C-MYC as well as one of the major E3 ligase components targeting C-MYC, from interacting with and ubiquitinating C-MYC in feedback.

Silica Glass-Based Droplet Generation Microfluidic Chips Enabled by Femtosecond Laser: Simulation Analysis, Controllable Fabrication, and Performance Evaluation.

Microdroplet technology is widely used in biomedicine, material synthesis, and the petrochemical industry due to its unique biological and mechanical properties. Silica glass has emerged as a key substrate for droplet microfluidic chips because of its excellent physical, chemical, and biocompatible characteristics. However, its intrinsic hardness and brittleness create significant challenges in fabrication.

METTL3-mediated m6A modification of SLC7A11 enhances nasopharyngeal carcinoma radioresistance by inhibiting ferroptosis.

Radiotherapy is the primary treatment for nasopharyngeal carcinoma (NPC); nonetheless, radioresistance remains the leading cause of localized recurrence. Our study demonstrates a significant increase in the N6-methyladenosine (m6A) methylase METTL3 in NPC and other tumors. Mechanistically, METTL3 acts as an m6A methylase, enhancing the m6A modification of the solute carrier family 7 member 11 (SLC7A11) transcript, which increases its stability and expression, thereby inhibiting radiation-induced ferroptosis and ultimately inducing radioresistance in NPC.

HSF1 Mediates Palmitic Acid-Disrupted Lipid Metabolism and Inflammatory Response by Maintaining Endoplasmic Reticulum Homeostasis in Fish.

Fish oil (FO) is being progressively replaced by palm oil (PO), which is rich in palmitic acid (PA). However, our understanding of the effects of PA on fish and the underlying molecular mechanisms remains limited. Heat shock transcription factor 1 (HSF1) is a critical transcription factor involved in stress response, but whether it responds to the effects of PA in fish remains unknown.

Activation of prep expression by Tet2 promotes the proliferation of bipotential progenitor cells during liver regeneration.

Biliary epithelial cell (BEC)-derived liver regeneration in zebrafish exhibits similarities to liver regeneration in chronic liver injury. However, the underlying mechanisms remain poorly understood. Here, we identified a serine peptidase called prolyl endopeptidase (Prep) as an indispensable factor during the BEC-derived liver regeneration process.

Effects of dietary fatty acids on growth performance, oxidation status, fatty acid and phospholipid profiles of razor clam Sinonovacula constricta.

Bivalves are a good source of lipid such as fatty acids and phospholipids, but little is known about how dietary fatty acids affect the fatty acid and phospholipid profiles of bivalves. In this study, the effects of dietary fatty acids on growth, oxidation status, fatty acid and phospholipid profiles of razor clams were investigated by employing microencapsulated feeds containing different oils (fish oil, FO; soybean oil, SO; flaxseed oil, FLO; coconut oil, CO; arachidonic acid-enriched oil, AO; olive oil, OO; and palm oil, PO). The results showed that the final weight of the razor clams fed FO enriched with eicosapentaenoic acid (EPA, C22:5n-3) and docosahexaenoic acid (DHA, C22:6n-3) was significantly higher than that of all other treatment groups.

X-scPAE: An explainable deep learning model for embryonic lineage allocation prediction based on single-cell transcriptomics revealing key genes in embryonic cell development.

In single-cell transcriptomics research, accurately predicting cell lineage allocation and identifying differences between lineages are crucial for understanding cell differentiation processes and reducing early pregnancy miscarriages in humans. This paper introduces an explainable PCA-based deep learning attention autoencoder model, X-scPAE (eXplained Single Cell PCA - Attention Auto Encoder), which is built on the Counterfactual Gradient Attribution (CGA) algorithm. The model is designed to predict lineage allocation in human and mouse single-cell transcriptomic data, while identifying and interpreting gene expression differences across lineages to extract key genes.

Clinical application of third-generation dual-source CT-based dynamic imaging reconstruction for pulmonary embolism imaging.

Background: To evaluate the clinical diagnostic value of third-generation dual-source CT for pulmonary embolism, focusing on the optimization of dual-source CT scanning with dynamic reconstruction in acute pulmonary embolism (PE) and various imaging manifestations.

Methods: Eighty-two patients with pulmonary embolism were enrolled and randomly divided into standard CT angiography (SCTA) and dynamic CT angiography (DCTA). DCTA patients were divided into dynamic CT angiography arterial phase (DCTAa), time phase Angiography reconstruction (TMIP-CTA), and 4D noise reduction TMIP-CTA according to the image reconstruction.

N6-methyladenosine-modified SRD5A3, identified by IGF2BP3, sustains cisplatin resistance in bladder cancer.

Resistance to cisplatin-based chemotherapy limits the clinical benefit to some bladder cancer patients, and understanding the epigenetic regulation mechanism of cisplatin (CDDP) resistance in bladder cancer from the perspective of N6-methyladenosine (m6A) modification may optimize CDDP-based treatments. The study identified SRD5A3 as an oncogene for bladder cancer and stabilized by a m6A reader, IGF2BP3, to sustain CDDP resistance. Our results revealed that the expression of SRD5A3 was elevated in human bladder cancer tissues and cell lines, and this elevation was more evident in CDDP-resistant T24 and 5637 cells.

Multifunctional Conjugated Polyphenol "Bridge" Capped the Upper Interface of Highly Efficient and Stable Inorganic Perovskite Solar Cells Fabricated in Air.

Carbon-based all-inorganic perovskite solar cells (C-PSCs) are promising alternatives compared with conventional organic-inorganic halide PSCs because of their good thermal stability. However, low carrier transport efficiency and energy level mismatch of the upper interface of the device limit the further improvement of power conversion efficiency (PCE), and all-inorganic perovskites are extremely sensitive to oxygen and humidity, which seriously damages the long-term stability of the device. Upper interface modification is an effective solution to improve the PCE and the stability of devices.

Identifying the genetic association between severe autoimmune type 2 diabetes and the risk of focal epilepsy.

Background: Observational studies suggested a bidirectional relationship between severe autoimmune type 2 diabetes and focal epilepsy. However, it remains debated whether and in which direction a causal association exists. This genetics-based study aimed to explore the relationships of severe autoimmune type 2 diabetes (T2DM) and focal epilepsy outcomes with two sample Mendelian randomization (TSMR) method.

Effects of Dietary Docosahexaenoic Acid (DHA) Levels on Growth Performance, Fatty Acid Profile, and NF-B/Nrf2 Pathway-Related Gene Expression of Razor Clam .

Dietary docosahexaenoic acid (DHA) is crucial for the optimal (Opt) growth of bivalves, but the precise dietary DHA requirement remains undetermined in bivalves. Our study identifies the optimal dietary DHA requirement for razor clam and demonstrates its effects on fatty acid profiles and gene expression related to inflammation and detoxification. Microencapsulated feeds with different DHA levels (DHA1-6 groups: 1.

Synthesis, preclinical assessment, and first-in-human study of [F]d-FET for brain tumor imaging.

Purpose: Tumor-to-background ratio (TBR) is a critical metric in oncologic PET imaging. This study aims to enhance the TBR of [F]FET in brain tumor imaging by substituting deuterium ("D") for hydrogen ("H"), thereby improving the diagnostic sensitivity and accuracy.

Methods: [F]d-FET was synthesised by two automated radiochemistry modules.

The efficacy and potential mechanism of the acupuncture treatment for allergic rhinitis: A systematic review and meta-analysis of data from animal models.

Objective: This study aimed to evaluate the efficacy of acupuncture in treating allergic rhinitis (AR) in animal models of the disease, and to explore the underlying mechanism of acupuncture in AR.

Methods: Related literature was retrieved from multiple databases, including China National Knowledge Infrastructure (CNKI), Wan Fang databases, SinoMed databases, VIP database, PubMed, EMBASE, the Cochrane Library, up to September 2023. The inclusion criteria were focused on animal experiments that investigated the effect of acupuncture therapy on animal models of AR.

YOLO-CFruit: a robust object detection method for fruit in complex environments.

Introduction: In the field of agriculture, automated harvesting of fruit has become an important research area. However, accurately detecting fruit in a natural environment is a challenging task. The task of accurately detecting fruit in natural environments is complex due to factors such as shadows, which can impede the performance of traditional detection techniques, highlighting the need for more robust methods.

Preclinical characterization of [F]D-LW223: an improved metabolically stable PET tracer for imaging the translocator protein 18 kDa (TSPO) in neuroinflammatory rodent models and non-human primates.

Positron emission tomography (PET) targeting translocator protein 18 kDa (TSPO) can be used for the noninvasive detection of neuroinflammation. Improved in vivo stability of a TSPO tracer is beneficial for minimizing the potential confounding effects of radiometabolites. Deuteration represents an important strategy for improving the pharmacokinetics and stability of existing drug molecules in the plasma.

First Report of Leaf Spot Caused by on Chard ( var. L.) in China.

Chard ( var. L.) is popular vegetable in China.

First Report of Causing Yellow Wilt on Chinese Cabbage in China.

Chinese cabbage (Brassica rapa L. ssp. pekinensis), in the family Brassicaceae, is a widely planted crop in China valued for its nutritional benefits.

S100A9-TLR4 axis aggravates dry eye through the blockage of autophagy.

This research focused on how upregulation of S100A9 contributed to the pathogenesis of the dry eye disease (DED) and whether S100A9 served as a promising therapeutic target in DED. Public single-cell RNA sequencing (scRNA-seq) data of a lacrimal gland excision (LGE) murine DED model was analyzed. LGE model was established and expression of protein was measured through immunofluorescence and Western blot.

Application of Nanomaterials and Related Drug Delivery Systems in Autophagy.

Autophagy, a lysosomal self-degradation pathway, plays a critical role in cellular homeostasis by degrading endogenous damaged organelles and protein aggregates into recyclable biological molecules. Additionally, it detoxifies extracellular toxic substances, including drugs and toxic materials, thereby preserving the stability of the intracellular environment. The swift progression of nanotechnology has led to an increased focus on understanding the relationship between nanomaterials and autophagy.