Chlorogenic acid induces ROS-dependent apoptosis in Fusarium fujikuroi and decreases the postharvest rot of cherry tomato.

Chlorogenic acid is a plant polyphenol with antioxidant and antimicrobial activities. Fusarium fujikuroi is a fungal pathogen that causes many vegetables and fruits, including tomato, to rot. The effects of chlorogenic acid on the development of Fusarium rot of cherry tomato fruit were examined in the present study.

Expression of miR-195 and MEK1 in patients with bladder cancer and their relationship to prognosis.

Objective: MAPK kinase 1 (MEK1) plays an important role in regulating cell proliferation and apoptosis through activation of the ERK/MAPK signaling pathway. It was found that the expression of miR-195 in bladder cancer was abnormally decreased, suggesting that miR-195 may affect the development of bladder cancer. In this study, we examined the expression of miR-195 and MEK1 in bladder cancer tissues and analyzed the relationship between miR-195 and MEK1 in cell proliferation and apoptosis in bladder cancer cells.

Curcumin Induces Oxidative Stress in , Resulting in a Reduction in Gray Mold Decay in Kiwifruit.

Curcumin exhibits efficient antimicrobial activity; nevertheless, its effect on the postharvest decay of fruit has not been examined. Here, effects of curcumin on the fruit gray mold of kiwifruit infected by were analyzed. Results demonstrated that curcumin induced reactive oxygen species (ROS) production and triggered apoptosis in hyphae.

[Genetic diagnosis for a pedigree affected with hereditary nephrogenic diabetes insipidus].

Objective: To explore the genetic basis for pedigree affected with hereditary nephrogenic diabetes insipidus (HNDI).

Methods: Next generation sequencing (NGS) with an osteology system gene panel was carried out for the proband. Suspected mutation was validated by Sanger sequencing of two relatives with similar symptoms and two unaffected relatives from the pedigree.

Distribution of arginine-vasopressin in the trigeminal, dorsal root ganglia and spinal cord of the rat; depletion by capsaicin.

We have measured arginine vasopressin in the neural lobe, the trigeminal ganglion (TG), dorsal root ganglia (DRG), spinal cord, trigeminal and sciatic nerves of the rat by radioimmunoassay. In control rats, the neural lobe contained 1600 pg/mg, the ganglia 52.5, 21.

Glutamate-immunoreactivity in the trigeminal and dorsal root ganglia, and intraspinal neurons and fibres in the dorsal horn of the rat.

Putative aspartergic and glutamatergic sensory neurons in the rat were identified by autoradiography and immunocytochemistry respectively. Approximately 3% of large L4 dorsal root ganglion neurons (diameter 18-52 microns) accumulated radiolabelled aspartate, whereas all satellite glia had high affinity for the amino acid. Glutamate-immunofluorescent (Glu-FITC) dorsal root ganglia neurons comprised 38.

Cytochemistry of the trigeminal and dorsal root ganglia and spinal cord of the rat.

1. The primary sensory neurones have been classified into large light (LLC), type A, small dark (SDC), type B and type C cells on the basis of size, ultrastuctural and immunocytochemical characteristics. 2.

A quantitative analysis of the interrelationships between subpopulations of rat sensory neurons containing arginine vasopressin or oxytocin and those containing substance P, fluoride-resistant acid phosphatase or neurofilament protein.

In rat L5 dorsal root ganglia 50% of neurons contained arginine vasopressin-like immunoreactivity and 38% oxytocin-like immunoreactivity, the oxytocin entirely coexisting with the arginine vasopressin. Staining of alternate mirror-image sections with RT97 (an antibody to neurofilament protein, and a marker for large light neurons) and with arginine vasopressin antiserum showed that the two were entirely complementary, thus establishing arginine vasopressin as a marker for all small dark neurons. Mirror-image staining also showed that neurons containing substance P-like immunoreactivity and those containing fluoride-resistant acid phosphatase activity were each contained within the arginine vasopressin-positive population.

Localization of chromatographically characterized oxytocin and arginine-vasopressin to sensory neurones in the rat.

Following treatment with colchicine 50-60% of all neurones in rat trigeminal and L5 spinal ganglia showed oxytocin (OXT)- and arginine-vasopressin (AVP)-like immunoreactivity. Further, OXT and AVP, together with their associated neurophysins, could be isolated from trigeminal ganglia by reversed-phase high-performance liquid chromatography followed by radioimmunoassay.

Localization of 5-hydroxytryptamine to neurons and endoneurial mast cells in rat sensory ganglia.

5-Hydroxytryptamine (5-HT) localization has been studied in the spinal and trigeminal ganglia of adult rats using immunofluorescence, peroxidase-antiperoxidase immunocytochemistry and [3H] 5-HT uptake radioautography, the latter two at the ultrastructural level. Endoneurial mast cells, identified by alcian blue staining, formed 8 and 14% of all mast cells and neurons in spinal and trigeminal ganglia respectively and had a median diameter of 7.6 microns.

Ultrastructural localization of [3H]dopamine in neurons of leech (Haemopis sanguisuga) abdominal ganglia.

Neurons in the cortex of the segmental ganglia of Haemopis sanguisuga accumulated [3H]DOPA in vitro. Electron microscopic autoradiography revealed deposits of silver grains over clusters of electron dense granules measuring 20-35 nM in diameter. [3H]Dopamine (DA) was localized in the giant glial cells.

Incorporation of [3H]2-deoxyglucose into glycogen in nervous tissues.

Mice were injected with [3H]2-deoxyglucose and after 1 h high molecular weight glycogen was extracted from brain, liver and muscle tissues. 1-2% of the total radioactivity in each tissue was recovered in the glycogen fraction. Isolated buccal ganglia of the pond snail, Planorbis, and isolated abdominal ganglia of the horse leech Haemopis, were exposed in vitro to [3H]2-deoxyglucose for 1 h.

The structure, distribution, and quantitative relationships of the glia in the abdominal ganglia of the horse leech, Haemopis sanguisuga.

The glial cells in abdominal ganglia of the horse leech Haemopis sanguisuga were studied by electron microscopy and analysed quantitatively to evaluate the suitability of this easily obtainable carnivorous species for physiological studies. Each abdominal ganglion contains eight giant glial cells, 12,000-14,000 small glial cells, and approximately 300 neurons. The giant glial cells constituted 44.

High resolution analysis of [3H]2-deoxyglucose incorporation into neurons and glial cells in invertebrate ganglia: histological processing of nervous tissue for selective marking of glycogen.

The 2-deoxyglucose (2-DG) autoradiographic technique, in conjunction with 3H-labelled 2-DG and glutaraldehyde fixation, was applied to the isolated ganglia of the leech and the snail in order to analyse its potential use for the understanding of energy utilization in these simple nervous systems. Approximately 50% of the label is retained in these tissues after histological processing, and the method can be satisfactorily applied at the subcellular level. In both species most neurons progressively accumulate radioactivity over 15 min-1 h, although to different extents.