An efficient and low-cost method for the determination of selenium in selenium-enriched tea by high-performance liquid chromatography coupled with 3,3'-Diaminobenzidine derivatization.

In this study, a novel analytical method was developed and validated for the quantification of selenium in selenium-enriched tea. The various valence states of selenium present in tea were converted to Se(IV) through oxidation and reduction reactions. To chemically react with Se(IV), 3,3'-Diaminobenzidine (DAB) was employed to produce Se-DAB derivatives.