Critical evaluation of heat extraction temperature on soluble microbial products (SMP) and extracellular polymeric substances (EPS) quantification in wastewater processes.

While soluble microbial products (SMP) and extracellular polymeric substances (EPS) in wastewater bioprocesses have been widely studied, a lack of standard quantification procedures make it difficult to compare results between studies. This study investigated the effect of temperature on SMP and EPS profiles for biological nutrient removal (BNR) sludges and aerobic membrane bioreactor sludge by adapting the commonly used heat extraction and centrifugation scheme, followed by colorimetric quantification of the carbohydrate and protein fractions using the phenol-sulfuric acid (PS) and the bicinchoninic acid (BCA) methods, respectively. To overcome known inconsistencies in colorimetry, total carbon (TC), total nitrogen (TN), and fluorometry analyses were performed in tandem.

sp. nov., isolated from conserved forages.

A Gram-stain-negative, long-rod-shaped and facultative aerobic bacterium, designated HB-1, was isolated from a round hay bale at the Kansas State University Beef Stocker Unit. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB-1 clustered within the genus and its closest relatives were A1-9 (98.0 %), YJ-T1-11 (98.

Long-Term Performance of a Pilot-Scale Gas-Sparged Anaerobic Membrane Bioreactor under Ambient Temperatures for Holistic Wastewater Treatment.

Concerns regarding ambient temperature operation, dissolved methane recovery, and nutrient removal have limited the implementation of anaerobic membrane bioreactors (AnMBRs) for domestic wastewater treatment. This study addresses these challenges using a pilot-scale gas-sparged AnMBR, with post-treatment recovery of dissolved methane and nutrients. Operating under ambient temperatures for 472 days, the AnMBR achieved an average effluent quality of 58 ± 27 mg/L COD and 25 ± 12 mg/L BOD at temperatures ranging from 12.

A comparative pilot-scale evaluation of gas-sparged and granular activated carbon-fluidized anaerobic membrane bioreactors for domestic wastewater treatment.

Two significantly different pilot-scale AnMBRs were used to treat screened domestic wastewater for over one year. Both systems similarly reduced BOD and COD by 86-90% within a 13-32 °C temperature range and at comparable COD loading rates of 1.3-1.