Grading the Severity of Damage to the Perijunctional Actomyosin Ring and Zonula Occludens-1 of the Corneal Endothelium by Ensemble Learning Methods.

In many epithelia, including the corneal endothelium, intracellular/extracellular stresses break down the perijunctional actomyosin ring (PAMR) and zonula occludens-1 (ZO-1) at the apical junctions. This study aims to grade the severity of damage to PAMR and ZO-1 through machine learning. Immunocytochemical images of PAMR and ZO-1 were drawn from recent studies on the corneal endothelium subjected to hypothermia and oxidative stress.

Automated Image Segmentation of the Corneal Endothelium in Patients With Fuchs Dystrophy.

Purpose: To perform segmentation of specular microscopy (SM) images of the corneal endothelium for comparing average perimeter length (APL) between Fuchs endothelial corneal dystrophy (FECD) patients and healthy subjects.

Methods: A retrospective review of clinical records of FECD patients and those with healthy endothelium was carried out to collect images of the endothelium. The images were segmented by modified U-Net, a deep learning architecture, followed by the Watershed algorithm to resolve merged cell borders (<5%).

Robust and computationally efficient superresolution algorithm.

Superresolution is the process of combining information from multiple subpixel-shifted low-resolution images to form a high-resolution image. It works quite well under ideal conditions but deteriorates rapidly with inaccuracies in motion estimates. We model the original high-resolution image as a Markov random field (MRF) with a discontinuity adaptive regularizer.