A PiggyBac mediated approach for lactoferricin gene transfer in bovine mammary epithelial stem cells for management of bovine mastitis.

The antibacterial and anti-inflammatory properties of lactoferricin have been ascribed to its ability to sequester essential iron. The objective of the study was to clone bovine lactoferricin () gene into PiggyBac Transposon vector, expression study in the bovine mammary epithelial stem cells (bMESCs) and also to determine the antimicrobial property of recombinant against bovine mastitis-causing organisms. The PiggyBac-LFcinB was transfected into bMESCs by electroporation and a three fold of LFcinB secretion was observed in the transfected bMESCs medium by ELISA assay.

Superficial adnexal lymphangioma.

An 11-year old girl presented with a painless fleshy lesion involving the plica, caruncle and medial bulbar and palpebral conjnctiva of the right eye since birth. The patient underwent debulking and cryotherapy. Biopsy showed lymphatic vessels lined with one flattened layer of endothelial cells devoid of smooth muscles or pericytes.

Self-inserted intraorbital foreign bodies.

We report an unusual case of self-mutilation by insertion of multiple foreign bodies in the orbit. A 26-year-old female psychiatric patient with a severe emotionally unstable personality disorder was reviewed in the ophthalmology clinic. She had allegedly inserted a metal staple into her right eye 2 days previously and was complaining of pain around the right eye.

Clinical research, comparison of the subjective visual function in patients with epiphora and patients with second-eye cataract.

Purpose: To assess the subjective visual disability of patients with epiphora and to compare the results with that of patients waiting for second eye cataract surgery.

Design: A prospective, randomised, questionnaire-based study.

Methods: Forty-six patients with epiphora listed for dacryocystorhinostomy and 50 patients having second eye cataract extraction were enrolled.

Ocular significance of intraventricular haemorrhage in premature infants.

Aim: To document ocular outcome in premature infants with intraventricular haemorrhages (IVH).

Methods: 68 preterm infants with IVH were examined.

Results: Mean gestational age was 28.

Ocular findings in glutaric aciduria type 1.

Purpose: To determine the nature and course of ocular abnormalities in glutaric aciduria (acidemia) type 1 (GA1).

Methods: Fifteen children with GA1 have been studied in the Republic of Ireland. A retrospective review of the records of the 6 children who died during their illness and prospective clinical examination of 9 survivors were performed.