[The estimation of platelet factor 3 activity in horses, cattle, sheep and pigs by the use of synthetic chromogenic tripeptide substrates].

The aim of this study was to investigate the platelet factor 3 activity in platelet-rich plasma of horse, cattle, sheep and pig, by the use of chromogenic tripeptide substrate H-D-Phenylalanyl-Pipecolyl-Arginyl-p-nitroanilide. Among species examined the highest activity of this factor was stated in pig, the lowest one in sheep. Chromogenic substrate test was 10 times more sensitive that Stypven clotting time test.

[Lipid peroxidation in dog lung microsomes].

The changes in lipid composition of dog lung microsomes during Fe3+/NADPH-induced peroxidation and correlation between malondialdehyde production and polyunsaturated fatty acids consumption were investigated. It was shown, that microsomal peroxidation causes phosphatidylcholine and phosphatidylethanolamine content decrease, with concomitant increase in lysophosphoglyceride content. Malondialdehyde production intensity was correlated with arachidonic and docosahexaenoic acid consumption.

[Effects of nifedipine and verapamil on cysteinyl leukotriene-induced contractions of the sheep uterus].

Both nifedipine and verapamil, the calcium channel blockers were tested to determine their anti-leukotriene properties in pig uterine strips. These drugs in concentrations ranging from 1.10(-7)M to 1.

[Effect of cysteinyl leukotrienes on uterine strips from pregnant and non-pregnant swine].

Cysteinyl leukotrienes C4, D4 and E4 elicited concentration-dependent contractile response of pregnant and non-pregnant pig uterine strips during incubation in De Jalon solution in concentrations between 1 x 10(-9)M and 1 x 10(-5)M for LTC4 and LTD4, and between 1 x 10(-8)M and 1 x 10(-5)M for LTE4. The maximum contractions elicited by leukotrienes were 77.5 +/- 3.

[Prostanoid precursors in the liver, lung and kidney of swine during ontogenesis].

Phospholipids and the levels of dihomo-gamma-linolenic, arachidonic and eicosapentaenoic acids in particular fractions of phospholipids in pig foetal liver, lung and kidney during ontogenesis, were investigated. A permanent rise in dihomo-gamma-linolenic and arachidonic acid content in phospholipid fractions of the above mentioned pig foetal internal organs was observed. In eicosapentaenoic acid level no changes were noted.

[Ontogenesis of antioxidant enzymes in swine].

The activities of catalase, peroxidase, glutathione peroxidase, glutathione reductase and superoxide dismutase as well as the levels of malondialdehyde during pig ontogenesis starting from 28 day of intrauterine life in some internal organs, foetal membranes and foetal fluids were investigated. The activities of enzymes thus investigated reached a peak in 2-day-old piglets. During foetal period as well as in 4-week-old sucklings and in mature pigs these activities were much lower.

[Lipids of pulmonary surfactant in the amniotic fluid of swine during ontogenesis].

Phospholipids and fatty acids in phosphatidylcholine and phosphatidylglycerol fractions of pulmonary surfactant isolated from pig amniotic fluid from the last trimester of pregnancy were investigated. The permanent rise in phosphatidylcholine and temporary increase in phosphatidylinositol contents in the two last weeks of pregnancy were observed. Phosphatidylcholine/sphingomyelin ratio was increasing beginning from the 85th day of pregnancy.

[Swine fetal membrane and myometrium lipids in ontogenesis].

Phospholipids and the levels of linoleic, dihomo-gamma-linolenic, arachidonic and eicosapentaenoic acids in its particular fractions in amnion, allantois and myometrium in pigs during pregnancy were investigated. A permanent rise in linoleic, dihomo-gamma-linolenic and arachidonic acid levels with advancement of gestation was observed. Eicosapentaenoic acid levels in such tissues, as well as the levels of above-mentioned acids in myometrium were greatly unchanged during the period thus analyzed.

Compensatory mechanisms in erythrocyte lipids in patients with atherosclerosis.

The quantitative composition of phospholipids and fatty acids of erythrocytes was investigated in patients with atherosclerosis. It was stated that the erythrocyte lipids of atherosclerotic patients contained smaller quantities of phosphatidylcholine and phosphatidylinositol, a significantly larger quantity of sphingomyelin, and higher sphingomyelin/phosphatidylcholine and cholesterol/phospholipid ratios. The existence of compensatory changes was stated, which was evident in the reduction of palmitic and stearic acids and the increase of linoleic and eicosatrienoic acids in erythrocyte phospholipids.

[Lipids of the liver and aorta of rabbits fed with cholesterol before and after surgical exclusion of the terminal segment of the ileum].

The experiments were carried out on 15 rabbits od common breed in 3 groups, K--control, 1--unoperated and 2--operated. The weight of the animals before operation oscillated from 3.8-4 kg.

The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis.

Concentrations of plasma triglycerides, cholesterol, total lipids and lipid peroxides in patients with atherosclerotic lesions of peripheral arteries, who were divided into groups according to the extent and intensity of the lesions, were estimated, as well as lipid peroxide levels in the arterial wall. Statistically highly significant increases of the estimated compounds were found in all groups in comparison with the controls. The existence of a positive correlation between the lipid peroxide concentration and other investigated components in plasma and between the lipid peroxide level in plasma and in the arterial wall was found.

Isolation and identification of prostaglandins of the E and F series in testes and semen of lowland-black-white breed of bulls.

The prostaglandins of E and F series were obtained from testes and semen of sexually mature bulls of lowland-black-white breed. From 1 g of fresh testes tissue we obtained 7.01 X 10(-9) M prostaglandin of the F series (PGF) and 20.

[Histochemical localization of prostaglandin synthetase in various organs in swine and guinea pigs].

The activity of prostaglandin synthetase was studied according to the histochemical method of Janszen and Nugteren (1971), in author's own modification, in the lungs, arteries and parotid gland of the pig and in the same organs of the guinea pig. Additionally, the seminal vesicles of guinea pig were subject to investigation. A high activity of this enzyme has been found in the endothelial cells of the arteries in both species and a lower activity in the non-striated muscles of the artery wall.

Occurrence and characteristics of sclerotic lesions in uterine arteries of sterile and multiparous pigs.

Sows and gilts of the Great White Polish (GWP) breed were classified into groups of 10 each: gilts 8 mo of age (A), sows over 1 yr old with an average litter size of five (BI) and eleven (BII), 2- to 3-yr-old sows with an average litter size of five (CI) and nine (CII), 4- to 6-yr-old sows with an average litter size of six (DI) and nine (DII). Gilts of group A were necropsied on d 10 of the estrous cycle while sows were necropsied 120 to 360 d postpartum. A blood sample obtained by vena cava puncture immediately before exsanguination was quantitated for total cholesterol, free cholesterol, cholesterol ester, total lipid, triglyceride, high and low density lipoprotein and chylomicrons.

Neutral lipids, phospholipids and higher fatty acids in bull's testes.

The lipid fractions were studied in the testicular tissue of mature bulls, of the lowland black-and-white breed. It was found that the main component of neutral lipids was cholesterol (48%) followed by triglycerides (24%), cholesterol esters (16%) and free fatty acids (12%). In cholesterol esters the main component was palmitic acid (41%) followed by oleic acid (22%), stearic acid (14%) and linoleic acid (14%).

Coronary arterial disease and atheromatosis in breeding sows.

The reported investigations were carried out on old breeding sows belonging to the Big White Polish Breed (BWPB) which were compared with a control group of young female pigs before fertilization, belonging to the same breed. In the perinatal period the animals were examined for presence of chronic ischaemic heart disease by clinical methods and ECG. In the interoestrus period determinations were performed in the serum of total cholesterol, free cholesterol, cholesterol esters, triglycerides, free fatty acids, total lipids and lipoprotein fractions.

[Lipids of the plasma, aortic wall and egg yolk of egg-laying hens after short-term feeding with atherogenic diet].

In laying hens of the Cornish breed about 1,8 kg of body weight, which, beside being fed abundantly for 4 days, were given 1 g of cholesterol and 1 g of pork fat in a piece directly into the crop, attempts were made to determine the level of fat compounds in blood plasma, in the wall of the abdominal aorta, and egg cell yolk. The control group was kept in similar conditions, receiving a bare piece of food. Blood was taken on empty stomach from the heart on heparin.

[Effect of partial ileal bypass on the liver lipid level in dogs on an atherogenic diet].

An attempt was undertaken to find out to what extent a partial ileal bypass in healthy dogs fed for 6 and 12 months on overloaded atherogenic diet with an addition of metizol influences the level of lipids and the development of atherosclerosis of the aorta. For the investigations 16 mongrels, both sexes, from 2 to 5 years old were used. an unoperated, control group consisted of 6 dogs, which were fed on the atherogenic diet for 12 months.

[Effect of atherogenic diet on the liver lipid level and development of aortic sclerosis in dogs].

Metabolic efficiency of the liver of 16 dogs, mixed breed, sexually mature, of both sexes was studied in a lasting experiment. The animals were kept in cages without a run, divided into 3 groups - k, A6 and A12. They were fed an atherogenic diet for 6 and 12 months.