Extracellular traps development in canine neutrophils induced by infective stage Toxocara canis larvae.

Neutrophils, a crucial element of the host defense system, develop extracellular traps against helminth parasites. Neutrophils accumulate around the larvae of Toxocara canis (T. canis) in the tissues of the organism.

Determination of extracellular traps structures from sheep polymorphonuclear leukocytes to Echinococcus granulosus protoscoleces.

It was aimed to detect extracellular traps structures from sheep polymorphonuclear leukocytes (PMN) after being confronted with Echinococcus granulosus protoscoleces in vitro. Also, the effect of cyst fluid was examined on the development of extracellular traps. At the end of the incubation for 1 h, the extracellular traps augmented with neutrophil elastase, histone (H3) and myeloperoxidase were visualized in the protoscoleces-PMN co-culture microscopically.

Vaccines Currently Available in the Field of Veterinary Parasitology.

Some parasite vaccines, developed for use in the veterinary field, are available in the market. Such vaccines usually contain live or attenuated parasites. Aside from these, a few parasite vaccines have also been prepared using recombinant technology.

Morphological and ecological features of some nematodes of the genus in marinka obtained from Fergana Valley, Uzbekistan.

The aim is to study the morphological and ecological features of some nematode species of the genus parasites of marinka fish in the Fergana Valley, Uzbekistan. and were detected as 5.3%, 7.

FELINE GIARDIASIS IN TURKEY: PREVALENCE AND GENETIC AND HAPLOTYPE DIVERSITY OF GIARDIA DUODENALIS BASED ON THE β-GIARDIN GENE SEQUENCE IN SYMPTOMATIC CATS.

Giardia duodenalis is a common zoonotic protozoan parasite with a broad host distribution. The main objectives of the present study were to determine the prevalence of giardiasis and to reveal the genetic and haplotype diversity of G. duodenalis in symptomatic cats in Turkey.

Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus.

Schmallenberg virus (SBV), discovered in Germany in 2011, causes congenital malformations in ruminants. Reverse-transcription real-time PCR (RT-rtPCR) assays based on various segments of SBV have been developed for molecular detection. We developed alternative RT-rtPCR assays for SBV detection to avoid earlier reported mutations and hypervariable regions of the S and M segments of the viral genome.

The first record on from mice in Turkey.

has been reported from both laboratory and wild rodents in worldwide. During routine care, soft consistency was observed in feces of Swiss albino mice. Motile spp.

Neutrophil isolation from feline blood using discontinuous Percoll dilutions.

Objective: Some studies have performed in vitro neutrophil isolation from feline blood. The major limiting factor for these studies is the small volume of blood that can be collected without development of potentially life-threatening complications. In the present study we attempted neutrophil isolation from feline venous blood samples using discontinuous Percoll gradients.

Comparison of Picogreen and Sytox Orange Stains in Quantitative Analysis of Extracellular Trap Formed against Toxoplasma gondii in Polymorphonuclear Leukocytes from Sheep.

Objective: The present study aimed to compare the effectiveness of two extracellular DNA dyes (Sytox Orange and PicoGreen) in quantitative measurement of the DNA that forms the backbone of the extracellular trap structures in vitro. Toward this aim, the co-culture of polymorphonuclear leucocytes (PMNs) and Toxoplasma gondii tachyzoites isolated from sheep was selected as model.

Methods: T.

Seroprevalence of Neospora caninum in Dairy Cattle Raised in Çiçekdaği District of Kırşehir Province.

Objective: Neospora caninum is one of the most important causes of abortion in cattle worldwide and causes significant economic losses in the meat and dairy industries. This study aimed to determine the seroprevalence of N. caninum in dairy cattle raised in Çiçekdağı district of Kırşehir province.

Prevalence of Haemoproteus spp. in Tumbler Pigeons (Columba livia domestica) in Kirikkale Province, Turkey.

Objective: Haemoproteus spp. are common blood parasites of pigeons. They have been reported in pigeons in many regions worldwide, including Turkey.

Role of NETs in the difference in host susceptibility to Toxoplasma gondii between sheep and cattle.

The main aim of this study was to compare extracellular traps (NETs) formation by polymorphonuclear neutrophils (PMNs) of cattle and sheep when exposed to T. gondii tachyzoites in vitro. The effects of parasite concentrations and different incubation periods on NETs development in cattle and sheep PMNs were studied.

Prevalence of Intestinal Parasites in Cats and Their Importance in Terms of Public Health.

Objective: This study aimed to detect the prevalence of intestinal parasites in cats by fecal examination.

Methods: A total of 100 fecal samples were collected from owned and stray cats in the Kırıkkale province. Epidemiological data on the sampled cats (e.

Netosis: Alternative Defense Method Used by Neutrophils to Fight Pathogen.

Neutrophils form the first line of defense of the innate immune system in organisms. They use three fighting methods to combat pathogens: phagocytosis, degranulation, and netosis. In this review, we aim to provide information about netosis.

Prevalence of intestinal parasites in hamsters and rabbits in some pet shops of Turkey.

Objective: In this study, we aimed to determine the parasite species carried by hamsters and rabbits purchased from some commercial pet shops in Turkey.

Methods: For this purpose, the fecal samples of clinically healthy Syrian hamsters, dwarf hamsters, and crossbred rabbits were collected from 22 pet shops randomly selected in Ankara and Kirikkale provinces, located in Central Anatolia Region of Turkey. The fecal samples were examined with centrifuge flotation technique using saturated salt solution.

[Seroprevalence of Toxoplasma gondii in sheep in Silopi district by using indirect fluorescent antibody test (IFAT)].

Objective: In the present study, it was aimed to detect seropositivity of T. gondii in sheep raised in the Silopi district.

Methods: For this aim, blood samples were obtained from 100 female Hamdani sheep.

In-vivo efficacy of toltrazuril on experimentally induced Toxoplasma gondii tissue cysts in lambs: a novel strategy for prevention of human exposure to meat-borne toxoplasmosis.

The aim of the present study was to investigate in vivo efficacy of toltrazuril on Toxoplasma gondii tissue cysts following induction of chronic toxoplasmosis in 4-week-old lambs (n=27) by inoculation of 1×10(5) T. gondii ME 49 strain oocysts (day 0). Beginning at the 15th day after inoculation, lambs in Group T20 and Group T40 were given toltrazuril orally 2 times, once every week (Baycox 5%, Bayer Animal Health) at a dose of 20 mg/kg and 40 mg/kg, respectively.

Antiparasitic efficiency of Artemisia absinthium on Toxocara cati in naturally infected cats.

Objective: The first aim of the present study was to determine the efficiency of A. absinthium extract on cats naturally infected with Toxocara cati. The second aim was to determine the efficiency of the extract on the embryonic development of T.

[The effect of different temperatures on viability of Aelurostrongylus abstrusus first stage larvae in faeces of cats].

Aelurostrongylus abstrusus lives in the respiratory bronchioles and alveolar canals in the lungs of the cat and causes parasitic pneumonia. In present study, the effect of different temperatures on the viability of first stage larvae in feces of cats naturally infected with A. Abstrusus was investigated.

Seroprevalence of Bartonella vinsonii subsp. berkhoffii in urban and rural dogs in Turkey.

The seroprevalence of Bartonella vinsonii subsp. berkhoffii was investigated in stray urban dogs and shepherd and farm guard dogs from rural areas sampled from 10 provinces of Turkey. Sera from 855 dogs were examined for the presence of anti-B.

Multivesicular cysts in cattle: characterisation of unusual hydatid cyst morphology caused by Echinococcus granulosus.

Echinococcus granulosus, the causative agent of cystic echinococcosis, not only often causes unilocular cysts in intermediate hosts, but also in rare cases induces formation of multivesicular cysts which have similar morphology to alveolar cysts. The aim of the present study was to characterise multivesicular and unilocular hydatid cysts in cattle using morphologic and molecular diagnostic tools. Multivesicular cysts were detected in 4 out of 1255 slaughtered cows.

The detection of Echinococcus granulosus strains using larval rostellar hook morphometry.

The purpose of this study was to determine the morphometrical characteristics of the larval hooks of Echinococcus granulosus in Turkey. The number of rostellar hooks (NH) and the total length of long blades, length of the blade of the long hooks, the total length of small blades and the length of the blade of the small hooks were measured in sheep and cattle isolates. The principal component and discriminant function analyses were used to analyze the data.

[Ceratothoa oestroides (Risso, 1826) (Isopoda: Cymothoidae) in black seabream from the Northern Aegean Sea].

Ceratothoa oestroides (Risso, 1826), is an ectoparasite found in a variety of fish species. This parasite was detected on caudal pedoncule of black seabram (Spondyliosoma cantharus) hunted in Balikesir. This is the first report of C.

Seroprevalence of Neospora caninum and coexistence with Toxoplasma gondii in dogs.

Neospora caninum is a protozoal agent causing abortion and infertility problems in dairy cattle. The objective of the present study was to detect the seroprevalence of N.caninum in dogs and its co-existence with Toxoplasma gondii in some rural and urban regions of Kirikka-le province.