Caffeine and 2-Aminoethoxydiphenyl Borate (2-APB) Have Different Ability to Inhibit Intracellular Calcium Mobilization in Pancreatic Acinar Cell.

Inositol 1,4,5-trisphosphate receptors (InsP(3)Rs) modulate Ca(2+) release from intracellular Ca(2+) store and are extensively expressed in the membrane of endoplasmic/sarcoplasmic reticulum and Golgi. Although caffeine and 2-aminoethoxydiphenyl borate (2-APB) have been widely used to block InsP(3)Rs, the use of these is limited due to their multiple actions. In the present study, we examined and compared the ability of caffeine and 2-APB as a blocker of Ca(2+) release from intracellular Ca(2+) stores and Ca(2+) entry through store-operated Ca(2+) (SOC) channel in the mouse pancreatic acinar cell.

Effects of gonadal steroid hormones on amylase secretion in pancreatic lobules of rats.

Objectives: Because functional changes of the pancreas during the ovarian cycle are not fully understood, effects of gonadal steroid hormones on pancreatic amylase content and secretion were investigated.

Methods: The estrus cycle of female rats was confirmed by vaginal smear. To mimic the estrus or the diestrus, estradiol 17beta (25 microg/kg) or progesterone (50 mg/kg) was subcutaneously injected to ovariectomized rats once daily for 2 days.