[Interactions of various cationic detergents with cholinesterase of horse blood serum].

Cetyltrimethyl ammonium and cetylpyridinium, both being cationic detergents, have been studied for their effect on the catalytic activity of horse blood serum cholinesterase (BuHChE) in reactions of hydrolysis of carbonic acid esters. It is shown that the detergents tested are reversible competitive inhibitors of the reaction of butyryl cholinesterase hydrolysis of butyryl choline, a specific cationic substrate, but in this case they activate enzymic hydrolysis of alpha-naphthylacetate, a nonspecific neutral substrate. Values of constants, describing enzyme binding with a detergent, are estimated both by the degree of inhibition of enzymatic hydrolysis of butyryl choline and by the degree of activation of enzymatic hydrolysis of alpha-naphthylacetate and are practically equal.

Effect of orthotopic transplantation of liver on systemic and splanchnic hemodynamics in conscious rat.

The long-term cardiovascular effects of orthotopic liver transplantation (OLT) were studied in conscious Lewis rats with a radioactive microsphere technique. Three months after OLT with an all-suture technique for graft revascularization (s-OLT), all hemodynamic parameters were similar to control. OLT with "cuffs" fitted to the portal vein and infrahepatic inferior vena cava (c-OLT) led to prominent hemodynamic disturbances including 1) hyperkinetic circulation with increased cardiac index (CI; 22%; P < 0.

[Change in the functional activity of the blood complement system after administration of adrenaline to animals].

Epinephrine given to non-inbred male albino rats in a pharmacological dose of 1 mg/kg was studied for effects on their serum complement. Following 5 min of its administration, the complement activity both of blood overall and individual parameters reduced by 0.4-0.

[Mutagenic effect of oxidizing agents on the thermally-induced prophage lambda cI857: effect of a system of oxidative stress].

The lethal and mutagenic effects of hydrogen peroxide H2O (2-20 mM), cumene hydroperoxide (0.2-2.0 mM), and potassium permanganate KMnO4 (0.

Regression of bile duct damage and bile duct proliferation in the non-rearterialized transplanted rat liver is associated with spontaneous graft rearterialization.

The aim of this study was to investigate the long-term consequences of non-rearterialization of the graft in rat liver transplantation. Liver transplantation with (AOLT) and without graft rearterialization (NOLT) was performed in anesthetized male Lewis rats. Quantitative morphometry and semiquantitative histopathology of the liver were performed at various times after operation.

[The interdigestive phasic motility of the gallbladder and bile excretion in healthy subjects and in patients with duodenal peptic ulcer].

Interdigestive phasic motility of the gallbladder and bile excretion into the duodenum were studied in 100 patients with duodenal ulcer (DU) and 20 healthy subjects. Gall bladder motility and bile excretion in both healthy and DU subjects directly depended on interdigestive phasic activity of the duodenum and gastric antrum. In health, gallbladder volume reached its maximum within termination of phase II, minimum in the end of phase III-beginning of phase I.

[Mutant alleles for radioresistance form the Escherichia coli strain Gam(r)444): cloning and preliminary characteristics].

Mutant alleles Gamr, which are able to increase the resistance to radiation of Escherichia coli wild-type cells, were cloned from the hyperradioresistant mutant Gamr444 on a plasmid mini-Mu vector MudII4042. The influence of recombinant plasmids on the sensitivity of wild-type and mutant (recA and htpR) cells to gamma-irradiation was studied. It was shown that the enhanced resistance of the Gamr444 strain to radiation was caused by mutations of two different classes, dominant and recessive.

[A kinetic study of the blood serum cholinesterase activity in the freshwater fish Abramis ballerus].

In reaction of hydrolysis of choline and thiocholine esters of carbonic acids at 25 degrees C, cholinesterase activity of the blood serum from the fish A. ballerus has been studied by modified Ellman's method and potentiometric titration method. The activity is maximal in pH region 7.

[Estimation of anticholinesterase activity in reversible inhibitors of cholinesterase hydrolysis].

The kinetic analysis of cholinesterase interaction with reversible inhibitors was carried out. It has shown that the existing methods for definition of the type of reversible inhibition of enzyme reactions are not reliable. For example, mixed inhibition with the correlation between the competitive inhibition constant (Kl) and noncompetitive inhibition constant (K'i) less than 0.

[The effect of stretching on mechanical chemical characteristics of tissues from elongated extremities].

A number of biochemical patterns of blood serum were used as markers of the major components of cartilage and muscle tissues, which exhibited adequately the property of responses occurred in these tissues during elongation of an extremity by the Ilizarov's procedure. The content of these components was shown to correlate in dog blood serum and tissues. At the same time, the rate of hydration, concentration of proteoglycans and elastic properties of the joint cartilage were studied.

Plants transformed with a cistron of a potato virus Y protease (NIa) are resistant to virus infection.

An oligonucleotide carrying signals for translation initiation in plants was engineered upstream to a cDNA clone containing nucleotides 5812-7260 of the potato virus Y (PVY) genome. This fragment contains all but the first 100 5' terminal bases of the cistron encoding one of the PVY proteases (NIa) as well as the first 251 bases of the next cistron (NIb). Nicotiana tabacum cv.

[Hemodynamic changes in waking rats with acute streptozotocin diabetes after administration of the combined V2/V1 vasopressin antagonist].

The hemodynamic effect of combined V2/V1 arginine vasopressin (AVP) antagonist in conscious rats with acute 24 hour streptozotocin (STZ)--induced diabetes was studied using the microsphere technique. The rats were made diabetic with a single intravenous injection of STZ (60 mg/kg). One day after STZ administration the hemodynamic parameters in the experimental animals were measured before and 10 minutes after AVP antagonist injection (50 mg/kg, i.

[Cardiac output and its distribution in waking rats with acute streptozotocin diabetes].

Cardiac output and its regional distribution were studied in conscious rats with acute 24-hour streptozotocin (STZ)-induced diabetes using the labeled microsphere technique. Rats were made diabetic with a single intravenous injection of STZ (60 mg/kg). One day post-STZ all rats were divided into two groups in accordance with the results of urine analysis: rats with ketonuria (n = 6) and rats without ketonuria (n = 7).

[The smooth-muscle adenylate cyclase system of invertebrate and vertebrate animals as the target of glucagon action].

Stimulatory effect, as well as potentiation of the latter by guanine nucleotides, of mammalian glucagon on adenylate cyclase system from plain muscles of bivalve, gastropod molluscs, holothurians and ascidians have been revealed. The same effects were found in tissues of vertebrate animals (human myometrium, aorta and liver of rats, stomach and liver of chicks). These data indicate effective coupling of all the components of glucagon-sensitive adenylate cyclase system in plain muscles of all the investigated animals.