Complete genome sequence of the giant virus OBP and comparative genome analysis of the diverse ΦKZ-related phages.

The 283,757-bp double-stranded DNA genome of Pseudomonas fluorescens phage OBP shares a general genomic organization with Pseudomonas aeruginosa phage EL. Comparison of this genomic organization, assembled in syntenic genomic blocks interspersed with hyperplastic regions of the ΦKZ-related phages, supports the proposed division in the "EL-like viruses," and the "phiKZ-like viruses" within a larger subfamily. Identification of putative early transcription promoters scattered throughout the hyperplastic regions explains several features of the ΦKZ-related genome organization (existence of genomic islands) and evolution (multi-inversion in hyperplastic regions).

Molecular cloning of a xylosyltransferase that transfers the second xylose to O-glucosylated epidermal growth factor repeats of notch.

The extracellular domain of Notch contains epidermal growth factor (EGF) repeats that are extensively modified with different O-linked glycans. O-Fucosylation is essential for receptor function, and elongation with N-acetylglucosamine, catalyzed by members of the Fringe family, modulates Notch activity. Only recently, genes encoding enzymes involved in the O-glucosylation pathway have been cloned.

[Sepsis in patients with intracranial hemorrhage: incidence and influence on outcome].

The aim of the study is to analyze sepsis and septic shock incidence and their influence on the outcome in critically ill patients with intracranial hemorrhage. Sepsis incidence (33,7%) and septic shock incidence (18,6%) in the patients studied did not depend on intracranial hemorrhage etiology. Septic complications led to higher mortality which was 22,8% in patients with sepsis and 74,4% in patients with septic shock.

[Hyperbaric oxygenation influence on intracranial pressure in patents with intracranial hemorrhage receiving mechanical ventilation].

The study focuses on hyperbaric oxygenation (HBO) influence on the intracranial pressure (ICP) in patients with intracranial hemorrhages. Forty eight hyperbaric oxygenation sessions in 21 mechanically ventilated patients with intracranial hemorrhages were analyzed. HBO was accompanied by ICP increase in 31,3% of cases.

[Bacteriophage phi297--the new species of temperate phages Pseudomonas aeruginosa with a mosaic genome: potential use in phagotherapy].

The genome of halo-forming temperate Pseudomonas aeruginosa phage phi297 and lytic activity of its virulent mutant were studied. A mosaic structure was revealed for phi297 genome by its complete sequencing. The phi97 genome was partly homologous to the genomes of phages D3 and F116.

Genomic and proteomic characterization of the broad-host-range Salmonella phage PVP-SE1: creation of a new phage genus.

(Bacterio)phage PVP-SE1, isolated from a German wastewater plant, presents a high potential value as a biocontrol agent and as a diagnostic tool, even compared to the well-studied typing phage Felix 01, due to its broad lytic spectrum against different Salmonella strains. Sequence analysis of its genome (145,964 bp) shows it to be terminally redundant and circularly permuted. Its G+C content, 45.

[Morphological, clinical, diagnostic features and the treatment of patients with giant brain aneurisms].

Retrospective analysis of treatment outcomes of 89 patients with GIAs, treated in Sklifosovsky Emergency Care Institute from 01.01.1992 till 30.

[Surgical treatment of cranioorbital injuries combined with brain trauma].

Cranioorbital injuries (COI) are combined with brain trauma in 45-53% of patients and in 21--24% of cases require surgical treatment. Aim of this study was to evaluate the results of surgical treatment of patients with COI in acute period of traumatic brain injury (TBI). Among 4078 patients with TBI admitted to neurosurgical department of Sklifosovsky Institute of Emergency Medicine, 428 (10,5%) had COI.

Analysis of marker expression in porcine cell lines derived from blastocysts produced in vitro and in vivo.

The present study was designed to extensively characterize cell lines derived from porcine blastocysts by several methodical approaches, including morphological observation, cytogenetic analysis, estimation of alkaline phosphatase activity and detection of specific marker expression at the mRNA/protein level. A comparison was made between the properties of cell lines isolated from in vivo- and in vitro-obtained blastocysts. Our results showed that 57.

Point mobility of a cylindrical plate incorporating a tapered hole of power-law profile.

The paper describes the results of experimental measurements of point mobility carried out on circular plates containing tapered holes of quadratic power-law profile with attached damping layers. The obtained results are compared to the developed numerical model, as a means of validation. The profiles of the tapered hole in the plates are designed to replicate near zero reflection of quasi-plane waves from a tapered hole in geometrical acoustics approximation, also known as acoustic black hole effect.

The T7-related Pseudomonas putida phage φ15 displays virion-associated biofilm degradation properties.

Formation of a protected biofilm environment is recognized as one of the major causes of the increasing antibiotic resistance development and emphasizes the need to develop alternative antibacterial strategies, like phage therapy. This study investigates the in vitro degradation of single-species Pseudomonas putida biofilms, PpG1 and RD5PR2, by the novel phage ϕ15, a 'T7-like virus' with a virion-associated exopolysaccharide (EPS) depolymerase. Phage ϕ15 forms plaques surrounded by growing opaque halo zones, indicative for EPS degradation, on seven out of 53 P.

[Genome instability of Pseudomonas aeruginosa phages of the EL species: examination of virulent mutants].

The article continues a study of pseudolysogeny in Pseudominas aeruginosa infected with phiKZ-like phages of the EL species. Analysis was performed for several newly isolated virulent mutants of EL phages (EL and RU) that were virulent (capable of causing lysis of bacteria infected with the wild-type phage) and a lower extent of opalescence of negative colonies (NCs). Wile-type recombinants were detected in crosses of virulent mutants of phages EL and RU to confirm the polygenic control of virulence.

A genetic map of Xenopus tropicalis.

We present a genetic map for Xenopus tropicalis, consisting of 2886 Simple Sequence Length Polymorphism (SSLP) markers. Using a bioinformatics-based strategy, we identified unique SSLPs within the X. tropicalis genome.

[TL, the new bacteriophage of Pseudomonas aeruginosa and its application for the search of halo-producing bacteriophages].

The properties of new virulent bacteriophage TL of Pseudomonas aeruginosa belonging to the family Podoviridae (genome size of 46 kb) were investigated. This bacteriophage is capable of lysogenizing the bacterial lawn in halo zones around negative colonies (NC) of other bacteriophages. TL forms large NC, that are hardly distinguishable on the lawn of P.

[Nonspecific adaptive reactions blood in chronic brain ischemia].

Clinical and experimental studies were under way to examine nonspecific adaptive reactions of blood in chronic brain ischemia. The electrophoretic mobility of red blood cells and differential blood count were studied in animals (rats) when simulating local and total brain ischemia, as well as in patients with stages I, II, and III dyscirculatory encephalopathy. General mechanisms responsible for changing the adaptive reactions of blood were found in varying chronic brain ischemia in the experiments and clinical observations.

Acid-promoted synthesis of per-O-sulfated fucooligosaccharides related to fucoidan fragments.

The synthesis of per-O-sulfated derivatives of di-, tetra-, hexa-, octa-, dodeca-, and hexadecafucosides related to natural fucoidans of different types has been performed with the use of previously reported acid-promoted protocol for per-O-sulfation of polyols by SO(3) complexes. During the treatment of (1→3)-linked oligofucosides under these conditions with the promotion by TfOH, the unusual rearrangement of the reducing pyranose residue into furanose one was observed. To avoid the formation of rearrangement by-products, the use of a series of strong acids as promoters of sulfation of large oligofucosides was studied and the improved protocol was developed based on the use of TFA instead of TfOH.

[Levels of S100β protein in patients with mild traumamic brain injury].

The prospective study of plasma S100β protein levels in 19 patients with mild traumamic brain injury treated with conservative methods was carried out. The S100β protein levels were measured on the admission of the patient and after 24 and 48 h. The level of S100β increased above normal values (>0,105 mkg/l) in 11 patients (58%) in the first 6 h after the trauma.

Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes.

The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T. vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes.

[Brain cryodestruction in mammals in vivo].

26 animals (13 pigs and 13 rabbits) were involved in the study. Liquid nitrogen cryodestruction experimental  machine was used. The following measurements and investigation were performed: invasive temperature measurement, intraoperative ultrasound, magnetic resonance imaging and pathomorphologic assessment.

[Effect of a model of the H-component of a typical magnetic storm on early ontogenesis in Daphnia magna Straus].

The effect of a model of the H-component of a typical magnetic storm on the early ontogenesis of Daphnia magna Straus at 21 and 23 degrees C has been studied. It was shown based on the rates of the early ontogenesis that the effects of the model magnetic storm from the sudden onset of the storm to its end differ from the effects of the model magnetic storm from the recovery phase to the end of the storm. The effects of the model magnetic storm depended on temperature.

[Changes in the electrophoretic mobility of erythrocytes by the action of low-intensity pulse magnetic field].

It has been shown that the exposure of "intact" rats and human blood to low-intensity pulse magnetic field leads to similar changes in the electrophoretic mobility of erythrocytes, which may be connected with the modification of the spectrum of their membrane proteins.

[Systemic hemodynamic disorders in critically ill patients with intracranial hemorrhages].

The paper analyzes systemic hemodynamic disorders in 45 victims of severe brain injury and patients with nontraumatic intracranial hemorrhages. The incidence of hypovolemia in patients with nontraumatic intracranial hemorrhages and victims of severe brain injury is 65.4% and 73.

Selection and characterization of a multivalent Salmonella phage and its production in a nonpathogenic Escherichia coli strain.

We report the selection and amplification of the broad-host-range Salmonella phage phi PVP-SE1 in an alternative nonpathogenic host. The lytic spectrum and the phage DNA restriction profile were not modified upon replication in Escherichia coli Bl21, suggesting the possibility of producing this phage in a nonpathogenic host, contributing to the safety and easier approval of a product based on this Salmonella biocontrol agent.

[Adaptation reactions of rat blood exposed to low intensity electromagnetic radiation].

It is carried out research of action low-intensive electromagnetic radiations--low-intensive laser radiation and radiations of the highest frequency on normal animals and at modelling the stress-reaction, caused by introduction of adrenaline. Absence of effects of system of blood is noted at action low-intensive electromagnetic radiations on normal an organism and them correction action on alteration an organism, shown in restoration of the broken parameters--leukocyte the blood count, electrophoretic mobility of erythrocytes and phospholipide's structure of their membranes.

[Correction of endogenous intoxication syndrome in acute pancreatitis].

The endogenous intoxication syndrome was determined with chronic experiments to correlate to functional and metabolic disturbances of liver and intestines under acute pancreatitis. The remaxol use therapy corrects endogenous intoxication syndrome that based on hepato- and enteroprotective medicine effects.