Cytological and cytochemical properties of ten ascites tumors.

1. The cytological and cytochemical properties of the Ehrlich tetraploid and hyperdiploid, TA3 tetraploid and diploid, Krebs 2B, sarcoma 37, MC1M, 6C3HED and DBA lymphoma, and lymphoma #1 ascites tumors at a fixed interval after transplantation are described. 2.

Rabies neutralizing antibody response to different schedules of serum and vaccine inoculations in non-exposed persons.

Groups of adult humans, previously unexposed to rabies and with no history of rabies vaccination, were inoculated with different schedules of phenolized inactivated vaccine and Flury strain chicken-embryo vaccine, with or without one inoculation of hyperimmune serum. Serum specimens of the inoculated individuals were studied for antibody up to the 28th day following the first inoculation of the vaccines and serum. The results can be summarized as follows:1.

Studies with the electron microscope of virus-host relationships in Ehrlich ascites tumor cells. II. The localization and possible development of anopheles A virus within the endoplasmic reticulum of the host cell.

Evidence is presented suggesting that anopheles A virus in the Ehrlich ascites tumor cell is harbored and propagated within the endoplasmic reticulum which in this system consists of an extensive canalicular network. The organization and differentiation of endoplasmic reticulum within the cell are described and the significance of such a location of virus is discussed.

Studies with the electron microscope of virus-host relationships in Ehrlich ascites tumor cells. I. The identification and structure of anopheles A virus.

Sections of Ehrlich ascites tumor cells infected with anopheles A virus revealed particles in the cytoplasm, which were identified as the virus on the basis of their consistent size and internal structure; their high opacity in the electron beam after fixation with osmic acid; the localized occurrence, within a certain time interval following infection, of high concentrations of such particles within the cell; the absence of similar particles in uninoculated tumor cells and cells infected with other viruses, and the appearance of such particles in infected chorioallantoic membrane. The particles are spheroid, with a transparent 25 to 30 mmicro core, surrounded by an opaque complex shell 12 to 17 mmicro in thickness. Apparently "hollow," globular components of this shell have outer and inner diameters of approximately 12 mmicro and 5 to 7 mmicro respectively.

Laboratory data supporting the clinical trial of anti-rabies serum in persons bitten by a rabid wolf.

Five individuals severely exposed to rabies by wolf bite and treated with a course of phenolized vaccine alone showed no demonstrable antibodies in their sera until the nineteenth day following the start of treatment. Three of these five individuals died of rabies.On the other hand, twelve individuals similarly exposed, who received antirabies serum plus a course of phenolized vaccine, had demonstrable antibodies early in and throughout the period of observation.

Biological modification of rabies virus as a result of its adaptation to chicks and developing chick embryos.

This article describes experiments indicating a change in pathogenicity for laboratory animals of the Flury strain of rabies virus at high egg passages. Factors such as dilution of virus, number of egg passages, age of animals, and route of inoculation are taken into account. The results of the author's investigations indicate that living chick-embryo-adapted virus can be used both as a vaccine administered before exposure to rabies virus, and as an adjunct to antiserum in the protective treatment of animals after exposure.