A Perspective of Multi-Reflecting TOF MS.

Time-of-flight mass spectrometry (TOF MS) excels in rapid and high-sensitivity analysis, making it a cornerstone of analytical chemistry. But as sample complexity explodes in omics studies, so does the need for higher resolving power to ensure accurate results. Traditional TOF instruments face a challenge: achieving high resolution often requires a very large instrument.

Multireflecting gas chromatography-multireflecting time-of-flight mass spectrometer with high dynamic range.

Gas chromatography-mass spectrometry (GC-MS) is a cornerstone technique for analyses of volatile and semivolatile compounds, constantly evolving to meet new challenges. The adoption of time-of-flight mass spectrometers (TOFMS) has enhanced GC-MS with faster spectral acquisition and broader mass coverage, enabling rapid GC separations. Multireflecting TOFMS (MRT) further improves resolution compared to traditional single-reflecting TOFMS, facilitating the detection of minor analytes within complex matrices.

Collective dynamics and shot-noise-induced switching in a two-population neural network.

Neural mass models are a powerful tool for modeling of neural populations. Such models are often used as building blocks for the simulation of large-scale neural networks and the whole brain. Here, we carry out systematic bifurcation analysis of a neural mass model for the basic motif of various neural circuits, a system of two populations, an excitatory, and an inhibitory ones.

Modeling Known Sources of Mass Calibration Deviations in High Resolution ToF MS.

Modern time-of-flight mass spectrometer instruments exhibit excellent mass accuracy in the parts per billion range. At this level, mass calibration methods should consider potential distortions in the basic calibration. Factors which can cause distortions are to be under control.

Antibody response in elderly vaccinated four times with an mRNA anti-COVID-19 vaccine.

The humoral response after the fourth dose of a mRNA vaccine against COVID-19 has not been adequately described in elderly recipients, particularly those not exposed previously to SARS-CoV-2. Serum anti-RBD IgG levels (Abbott SARS-CoV-2 IgG II Quant assay) and neutralizing capacities (spike SARS-CoV-2 pseudovirus Wuhan and Omicron BA.1 variant) were measured after the third and fourth doses of a COVID-19 mRNA vaccine among 46 elderly residents (median age 85 years [IQR 81; 89]) of an assisted living facility.

Constructive role of shot noise in the collective dynamics of neural networks.

Finite-size effects may significantly influence the collective dynamics of large populations of neurons. Recently, we have shown that in globally coupled networks these effects can be interpreted as additional common noise term, the so-called shot noise, to the macroscopic dynamics unfolding in the thermodynamic limit. Here, we continue to explore the role of the shot noise in the collective dynamics of globally coupled neural networks.

Chronic Stress Alters Hippocampal Renin-Angiotensin-Aldosterone System Component Expression in an Aged Rat Model of Wolfram Syndrome.

Biallelic mutations in the gene encoding WFS1 underlie the development of Wolfram syndrome (WS), a rare neurodegenerative disorder with no available cure. We have previously shown that deficiency can impair the functioning of the renin-angiotensin-aldosterone system (RAAS). The expression of two key receptors, angiotensin II receptor type 2 () and bradykinin receptor B1 (), was downregulated both in vitro and in vivo across multiple organs in a rat model of WS.

Intramuscular mRNA BNT162b2 vaccine against SARS-CoV-2 induces neutralizing salivary IgA.

Intramuscularly administered vaccines stimulate robust serum neutralizing antibodies, yet they are often less competent in eliciting sustainable "sterilizing immunity" at the mucosal level. Our study uncovers a strong temporary neutralizing mucosal component of immunity, emanating from intramuscular administration of an mRNA vaccine. We show that saliva of BNT162b2 vaccinees contains temporary IgA targeting the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus-2 spike protein and demonstrate that these IgAs mediate neutralization.

Shot noise in next-generation neural mass models for finite-size networks.

Neural mass models is a general name for various models describing the collective dynamics of large neural populations in terms of averaged macroscopic variables. Recently, the so-called next-generation neural mass models have attracted a lot of attention due to their ability to account for the degree of synchrony. Being exact in the limit of infinitely large number of neurons, these models provide only an approximate description of finite-size networks.

The Expression of RAAS Key Receptors, and , Is Downregulated at an Early Stage in a Rat Model of Wolfram Syndrome.

Wolfram syndrome (WS) 1 is a rare monogenic neurodegenerative disorder caused by mutations in the gene encoding WFS1. Knowledge of the pathophysiology of WS is incomplete and to date, there is no treatment available. Here, we describe early deviations in the renin-angiotensin-aldosterone system (RAAS) and bradykinin pathway (kallikrein kinin system, KKS) observed in a rat model of WS ( KO) and the modulative effect of glucagon-like peptide-1 receptor agonist liraglutide (LIR) and anti-epileptic drug valproate (VPA), which have been proven effective in delaying WS progression in WS animal models.

Differential Contribution of Protein Factors and 70S Ribosome to Elongation.

The growth of the polypeptide chain occurs due to the fast and coordinated work of the ribosome and protein elongation factors, EF-Tu and EF-G. However, the exact contribution of each of these components in the overall balance of translation kinetics remains not fully understood. We created an in vitro translation system replacing either elongation factor with heterologous thermophilic protein from .

Noise-induced dynamical regimes in a system of globally coupled excitable units.

We study the interplay of global attractive coupling and individual noise in a system of identical active rotators in the excitable regime. Performing a numerical bifurcation analysis of the nonlocal nonlinear Fokker-Planck equation for the thermodynamic limit, we identify a complex bifurcation scenario with regions of different dynamical regimes, including collective oscillations and coexistence of states with different levels of activity. In systems of finite size, this leads to additional dynamical features, such as collective excitability of different types and noise-induced switching and bursting.

Reduction of the collective dynamics of neural populations with realistic forms of heterogeneity.

Reduction of collective dynamics of large heterogeneous populations to low-dimensional mean-field models is an important task of modern theoretical neuroscience. Such models can be derived from microscopic equations, for example with the help of Ott-Antonsen theory. An often used assumption of the Lorentzian distribution of the unit parameters makes the reduction especially efficient.

Draft Genome Sequence of Lactobacillus salivarius KZ-NCB, Isolated from Chicken Cecum.

Here, we report the draft genome sequence of strain KZ-NCB, which was isolated from the cecum of a healthy chicken from a poultry farm in Kazakhstan.

Peculiarities in Activation of Hydrolytic Activity of Elongation Factors.

Translational GTPases (trGTPases) belong to the family of G proteins and play key roles at all stages of protein biosynthesis on the ribosome. Unidirectional and cyclic functioning of G proteins is ensured by their ability to switch between the active and inactive states due to GTP hydrolysis accelerated by the auxiliary GTPase-activating proteins. Although trGTPases interact with the ribosomes in different conformational states, they bind to the same conserved region, which, unlike in classical GTPase-activating proteins, is represented by ribosomal RNA.

The role of timescale separation in oscillatory ensembles with competitive coupling.

We study a heterogeneous population consisting of two groups of oscillatory elements, one with attractive and one with repulsive coupling. Moreover, we set different internal timescales for the oscillators of the two groups and concentrate on the role of this timescale separation in the collective behavior. Our results demonstrate that it may significantly modify synchronization properties of the system, and the implications are fundamentally different depending on the ratio between the group timescales.

Self-association and picosecond dynamics in liquid dimethyl sulfoxide.

Dimethyl sulfoxide (DMSO) has been known for almost 150 years, but its properties as a solvent and reaction medium are far from being understood. In particular, association equilibriums in liquid DMSO have been characterized in just four papers, and the enthalpy of its self-association is unknown. The aims of this paper are to study self-association equilibriums in neat liquid DMSO at various temperatures by means of Raman spectroscopy, to find the enthalpy of self-association, and to solve the problem of hydrogen bonding in this liquid.

[Experimental base for construction alloy choice for implant-retained restorations].

The paper contains the results of experimental studies on galvanic features of "implant-construction alloy" contact pair. These results may be used as criteria for implant-retained restorations alloy choice.

Ejection of nanoclusters from gold nanoislet layers by 38 keV Au ions in the elastic stopping mode.

Total absolute yields of the ejected gold were obtained regardless of the type of the particles are--atoms, clusters, nanoclusters,--as well as absolute yields of gold nanoclusters, from nanoislet gold targets under bombardment by monoatomic gold ions at 45 degrees to the target surface with the energy 38 keV, i.e., in the "purely" elastic stopping mode -6 keV/nm up to the fluence of 4 x 10(12) cm2.

Perturbation of the tRNA tertiary core differentially affects specific steps of the elongation cycle.

The tRNA tertiary core region is important for both tRNA stability and activity in the translation elongation cycle. Here we report the effects of mutating each of two highly conserved base pairs in the tertiary core of Phe-tRNA(Phe), 18-55 and 19-56, on rate and equilibrium constants for specific steps of this cycle, beginning with formation of aminoacyl-tRNA.EF-Tu.

[Comparative assessment of the strength properties of the mucous membrane of the stomach in patients with peptic ulcer and the effect of quamatel on ulcer cicatrization in experiment].

The article reviews the research work of the authors on the strength properties of the mucous membrane of the stomach in patients with peptic ulcer and in experiment with quamatel application. Experiments were performed in laboratory animals and resected stomachs of patients with duodenal or stomach ulcer and complications requiring scheduled surgical treatment. The results of the research into the maximum tension (durability) of the stomach mucous membrane, antrum, and the periulcer area are described.

[Pulmonology of the individuals of call-up age].

In 1999-2001 400 patients in Saratov have been surveyed. The persons directed by the military-medical commissions of military registration and enlistment offices, have made 60.7% (243 patients).