Pentose phosphate pathway of glucose-6-phosphate oxidation in the liver of rats with alloxan diabetes on the background of melatonin administrations and variable photoperiod.

Objective: . Aim: To find out the influence of melatonin on the enzyme activities of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and transketolase in the liver of rats with alloxan diabetes under conditions of variable photoperiod.

Patients And Methods: Materials and Methods: Experiments were conducted on male outbred white rats weighing 180±10 mg.

Synthesis of ionizable lipopolymers using split-Ugi reaction for pulmonary delivery of various size RNAs and gene editing.

We present an efficient approach for synthesizing cationic poly(ethylene imine) derivatives using the multicomponent split-Ugi reaction to rapidly create a library of complex functional ionizable lipopolymers. We synthesized a diverse library of 155 polymers, formulated them into polyplexes to establish structure-activity relationships crucial for endosomal escape and efficient transfection. After discovering a lead structure, lipopolymer-lipid hybrid nanoparticles are introduced to preferentially deliver to and elicit effective mRNA transfection in lung endothelium and immune cells, including T cells with low toxicity.

A new era of targeting cystic fibrosis with non-viral delivery of genomic medicines.

Cystic fibrosis (CF) is a complex genetic respiratory disorder that necessitates innovative gene delivery strategies to address the mutations in the gene. This review delves into the promises and challenges of non-viral gene delivery for CF therapy and explores strategies to overcome these hurdles. Several emerging technologies and nucleic acid cargos for CF gene therapy are discussed.

Ytterbium 10-carboxyperylene-3,4,9-tricarboxylates for targeted NIR luminescent bioimaging.

Two new ytterbium coordination compounds Yb(HPTC)(HO) (Yb1) and Yb(HPTC)(Phen) (Yb2) were obtained using 10-carboxyperylene-3,4,9-tricarboxylate ion (HPTC) as a sensitizer. Both coordination compounds exhibited intense NIR-II luminescence upon excitation in the visible range and formed stable suspensions with nanoparticles of 50-70 nm in size in an aqueous solution of sodium alginate. Both complexes demonstrated non-toxicity up to at least 25 mg L in two cell cultures: cancer cells MCF7 and embryonic cells HEK293T - making them suitable for bioimaging.