Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1.

With recent advancements in gene editing technology using the CRISPR/Cas system, there is a demand for more effective gene editors. A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. Accordingly, compact CRISPR-Cas systems derived from various strains are discovered, among which Un1Cas12f1 is 2.

Predictive impact of clinical factors on chemosensitivity in advanced high-grade serous ovarian carcinoma according to chemotherapy response score.

The use of neoadjuvant chemotherapy (NAC) as a first-line therapy for advanced high-grade serous ovarian carcinoma (HGSOC) has increased. However, several studies have reported NAC-induced platinum resistance. This study aimed to evaluate the predictive impact of clinical factors on chemotherapy response score (CRS) and to select patients who would respond well to NAC.

Development of Predictive Model of Surgical Case Durations Using Machine Learning Approach.

Optimizing operating room (OR) utilization is critical for enhancing hospital management and operational efficiency. Accurate surgical case duration predictions are essential for achieving this optimization. Our study aimed to refine the accuracy of these predictions beyond traditional estimation methods by developing Random Forest models tailored to specific surgical departments.

Plasmon-Driven Gold Nanopillar Multiarrayed Gene Amplification Methodology for the High-Throughput Discrimination of Pathogens.

Molecular diagnosis limitations, including complex treatment processes, low cost-effectiveness, and operator-dependent low reproducibility, interrupt the timely prevention of disease spread and the development of medical devices for home and outdoor uses. A newly fabricated gold nanopillar array-based film is presented for superior photothermal energy conversion. Magnifying the metal film surface-to-volume ratio increases the photothermal energy conversion efficiency, resulting in a swift reduction in the gene amplification reaction time.