Identification of Novel Basil Downy Mildew Resistance Genes Using Comparative Transcriptomics.

Sweet basil ( L.) production is threatened by the oomycete pathogen , causing basil downy mildew (BDM). BDM-resistant cultivar 'Mrihani' (MRI) was identified in a germplasm screen, and bred with BDM-susceptible 'Newton' (SB22) to produce resistant cultivars, but the molecular mechanisms conferring resistance in MRI and the progeny remained unknown.

Biochemical characterization of the self-sacrificing p-aminobenzoate synthase from Nitrosomonas europaea reveals key residues involved in selecting a Fe/Fe or Mn/Fe cofactor.

A noncanonical route for p-aminobenzoate (pABA) biosynthesis in select bacteria utilizes a novel self-sacrificing heme oxygenase-like domain-containing oxidase/oxygenase (HDO) superfamily member. The recently characterized self-sacrificing pABA synthase from Chlamydia trachomatis ("CADD") requires manganese and likely employs a heterobimetallic Mn/Fe cofactor. A conserved active site tyrosine residue is cleaved from the protein backbone to serve as the substrate for pABA synthesis and a lysine residue is the amino group donor.

NAD+ prevents chronic kidney disease by activating renal tubular metabolism.

Chronic kidney disease (CKD) is associated with renal metabolic disturbances, including impaired fatty acid oxidation (FAO). Nicotinamide adenine dinucleotide (NAD+) is a small molecule that participates in hundreds of metabolism-related reactions. NAD+ levels are decreased in CKD, and NAD+ supplementation is protective.

Factors impacting loneliness in patients with serious life-limiting illness in the Emergency Medicine Palliative Care Access (EMPallA) study.

Background: Loneliness is a quality-of-life (QoL) concern for patients facing serious, life-limiting illnesses. Discerning risk factors of loneliness in palliative care patients allows providers to take preventative action and develop holistic treatment plans.

Methods: A planned sub-study of patients who completed the previously developed Three-Item Loneliness Scale upon enrollment into the multicenter, randomized clinical trial Emergency Medicine Palliative Care Access (EMPallA) with the objective of investigating the association of multimorbidity with loneliness in patients with late-stage illnesses.

Building capacity in patient-oriented research: reflections on cultivating relationships within a high-secure forensic mental health hospital.

Building trusting relationships is critical to the success of patient-oriented research. However, in high-secure forensic mental health settings, distrust, discrimination, and restrictive practices pose unique barriers to building relationships. This commentary explores the challenges, strategies, and lessons learned in fostering meaningful connections with forensic patients at a high-secure hospital.