In vitro response of human pathological hematopoietic cells to cladribine.

The influence of cladribine (2-chloro-2'-deoxyadenosine, CdA) on in vitro response of human acute lymphoblastic leukemia MOLT-4 cells, human histiocytic lymphoma U-937 cells, and human promyelocytic leukemia HL-60 cells, was determined using the MTT spectrophotometric and Beckman Coulter methods. Cell viability, cell volume and count were compared 24h and 48h after cladribine application at four concentrations--50 nM, 100 nM, 250 nM, and 500 nM. Different patterns of temporary changes in the viability, volume and count of pathological hematopoietic cells exposed to the action of CdA were found.

Mafosfamide as a new anticancer agent: preclinical investigations and clinical trials.

Mafosfamide (4-thioethane sulfonic acid salt of 4-hydroxy-cyclophosphamide, MAF) belongs to a new generation of the oxazaphosphorine agents. MAF is a cyclophosphamide analog which spontaneously degrades to 4-hydroxy-cyclophosphamide. The effects of MAF on various types of cancer cells were determined during preclinical investigations and clinical trials.

Comparative effects of new generation oxazaphosphorines on the size and viability of human acute myeloblastic leukemia cells.

Mafosfamide cyclohexylamine salt (D-17272), 4-hydro-peroxy-cyclophosphamide (D-18864), and beta-D-glucose-isophosphoramide mustard (D-19575, glufosfamide) are three new generation oxazaphosphorine agents. The aim of the present study was to compare the cell response to the action of these three oxazaphosphorines. The experiments were performed in vitro on human acute myeloblastic leukemia ML-1 cells.

[Parents and children in families with alcohol problems--anthropologic and preventive study].

The paper describes the proposition of solution of a difficult dilemma concerning the real role of parents in the development of children in families with alcohol problem (alcohol misuse and dependency of parents). From one side drinking parents are the cause of serious destruction of child health and development. From the other side we have the data about positive influence of strong bonds between children and their parents in alcohol use prevention (parenting as protective factor).

[Use of fructosamine serum level measurements for diagnosis of insulin-dependent diabetic in pregnancy].

In this study it has been tested the usefulness of serum glycosyl protein (fructosamine) to diagnose diabetic pregnant women requiring insulin therapy. The analysis was performed on the base of obtained results of glucose tolerance test, glycemia level in diurnal profile, fructosamine level and its relationship to albumin level and total protein amount in serum pregnant women with carbohydrates intolerance. Calculated specificity and sensitivity of above mentioned measurements was too low.