A 24-Hour Waste Audit of the Neuro ICU during the COVID-19 Pandemic and Opportunities for Diversion.

We conducted a series of 24-hour waste audits in a 20-bed pod of a Neurosciences Intensive Care Unit (Neuro ICU) during the COVID-19 pandemic to 1) determine the unit's waste generation practices, 2) calculate associated downstream greenhouse gas emissions, and 3) identify opportunities to reduce landfill waste and emissions. We collected and weighed municipal solid waste, regulated medical waste, and mechanical recycling. We then compared the current, "as-is" practices to an ideal, "should-be" model which adds the alternative waste and reprocessing streams of industrial composting, advanced recycling, and sterilization followed by reuse.

The small molecule Chicago Sky Blue promotes heart repair following myocardial infarction in mice.

The adult mammalian heart regenerates poorly after injury and, as a result, ischemic heart diseases are among the leading causes of death worldwide. The recovery of the injured heart is dependent on orchestrated repair processes including inflammation, fibrosis, cardiomyocyte survival, proliferation, and contraction properties that could be modulated in patients. In this work we designed an automated high-throughput screening system for small molecules that induce cardiomyocyte proliferation in vitro and identified the small molecule Chicago Sky Blue 6B (CSB).

A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2.

Background: Compartment boundaries are an essential developmental mechanism throughout evolution, designated to act as organizing centers and to regulate and localize differently fated cells. The hindbrain serves as a fascinating example for this phenomenon as its early development is devoted to the formation of repetitive rhombomeres and their well-defined boundaries in all vertebrates. Yet, the actual role of hindbrain boundaries remains unresolved, especially in amniotes.

Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System.

Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nervous system, provides a platform for the screening of chemical libraries that affect these processes, and offers a potential source of transplantable cells for regenerative approaches to neurological disease. However, defining protocols that permit a large number and high yield of neurons has proved difficult.