Adaptation of sea turtles to climate warming: Will phenological responses be sufficient to counteract changes in reproductive output?

Sea turtles are vulnerable to climate change since their reproductive output is influenced by incubating temperatures, with warmer temperatures causing lower hatching success and increased feminization of embryos. Their ability to cope with projected increases in ambient temperatures will depend on their capacity to adapt to shifts in climatic regimes. Here, we assessed the extent to which phenological shifts could mitigate impacts from increases in ambient temperatures (from 1.

The carboxyl-terminal domain of type VII collagen is present at the basement membrane in recessive dystrophic epidermolysis bullosa.

Recent studies showing that type VII collagen is a component of anchoring fibrils suggests that the absence of anchoring fibrils in recessive dystrophic epidermolysis bullosa may be due to a defect in the synthesis, secretion, and deposition of type VII collagen. That hypothesis is further supported by recent studies suggesting that monoclonal antibodies to type VII collagen do not react with the basement membrane in most patients. To investigate further, we examined skin from 12 patients by electron microscopy and by immunohistology and immunoelectron microscopy using a concentrated and purified monoclonal antibody to the carboxy-terminal domain of Type VII collagen.

Covalent attachment of 125I-beta nerve growth factor to its receptors on sympathetic neurons.

When 125I-beta nerve growth factor binds to sympathetic and sensory neurons, some labeled ligand is sequestered (becomes inaccessible to the external milieu) in a time- and energy-dependent manner. It would appear that the higher affinity receptor (type I) participates in this process to a greater extent than does the lower affinity receptor (type II) [ Olender and Stach , 1980; Olender et al., 1981].

Serum-free culture of chicken embryonic sensory ganglia in a balanced salt solution with nerve growth factor for periods up to two weeks.

Culture of embryonic chicken dorsal root ganglia for periods exceeding a week generally require serum supplementation and a trophic factor such as nerve growth factor. In this communication we describe the culture of chicken E-9 dorsal root ganglia for periods up to 2 weeks in a system that is composed only of a simple basic salts solution and 10 ng/ml (3.8 X 10(-10)M) beta nerve growth factor.

Interaction of [125I] beta nerve growth factor with acidic proteins.

We have demonstrated that the beta nerve growth factor will interact with various acidic proteins apparently nonspecifically. When 125I-labeled beta nerve growth factor at a concentration of 3.8 X 10(-10) M is incubated with an acidic protein at 2 mg/ml (4.