A Chromosome-Level Genome Sequence Reveals Regulation of Salt Stress Response in Mesembryanthemum crystallinum.

Salt stress disturbs plant growth and photosynthesis due to its toxicity. The ice plant Mesembryanthemum crystallinum is a highly salt-tolerant facultative crassulacean acid metabolism (CAM) plant. However, the genetic basis of the salt tolerance mechanisms in ice plants remains unclear.

Unfolding and refolding of GH19 chitinase Chi19MK with antifungal activity from Lysobacter sp. MK9-1 at low pH and high temperature.

The GH19 chitinase Chi19MK from Lysobacter sp. MK9-1 inhibits fungal growth. In this study, the thermal stability of Chi19MK was investigated in buffers of different pH.

[Long-term Reproducibility Evaluation of Fluoroscopy Dose Rate in Angiography Equipment].

Purpose: Diagnostic reference levels (DRLs) are defined as fluoroscopic dose rate measurements that are used for patient dose management in angiography. It is recommended that DRLs be measured at least once a year. This study aimed to evaluate the long-term fluctuations of fluoroscopic dose rate in an angiography system.

Automatic Hardy and Clapham's classification of hallux sesamoid position on foot radiographs using deep neural network.

Background: There is currently no deep neural network (DNN) capable of automatically classifying tibial sesamoid position (TSP) on foot radiographs.

Methods: A DNN was developed to predict TSP according to the Hardy and Clapham's classification. A total of 1554 foot radiographs were used for model development.

Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units.

During the production of bispecific antibodies (bsAbs), nonspecific pairing results in low yields of target bsAb molecules, an issue known as the "mispairing problem." Several antibody engineering techniques have been developed to overcome mispairing issues. Here, we introduce "bsAb by external pairing and excision" (BAPE), a novel chain pairing method that induces specific chain pairing by fusing external SpyCatcher/Tag and SnoopCatcher/Tag units.