Amide analogues of TSA: synthesis, binding mode analysis and HDAC inhibition.

The synthesis of new amide type histone deacetylase inhibitors is described, having an (R)-methyl substituent and a diene or saturated structure of the chain linking the hydroxamic acid and dimethylaminobenzoyl groups. The saturated compound shows stronger HDAC inhibition than the unsaturated analogue. Molecular modeling suggests that the flexibility of the linker chain is important for an optimal orientation of the dimethylaminobenzoyl group in the enzyme.

Prevalence of Leptospira interrogans serovar hardjo antibodies in milk in Belgian dairy herds.

Pooled milk samples were collected from 2000 Belgian dairy herds in the autumn of 1989. Antibodies against Leptospira interrogans serovar hardjo were detected in 9.2% of the herds, with the incidence being higher in the southern part of the country.

Comparative trial of xylazine and medetomidine as preanaesthetics prior to Na pentobarbital anaesthesia in dogs.

The effects of medetomidine [20 and 40 micrograms/kg of body weight (BWT) i.m.] followed by sodium pentobarbital were assessed and compared with the effects of xylazine (1 mg/kg of BWT i.

Field evaluation of an experimental albendazole pulse release bolus in the control of parasitic gastroenteritis in first-season grazing calves.

A field experiment is described which evaluates the use of an experimental albendazole pulse release bolus (E-bolus) on gastrointestinal parasitism. The bolus was administered at turnout to 9 first-season grazing calves and 9 animals were kept as controls on separate pasture. Dry summer months accounted for low infection levels in the calves; no cases of parasitic gastroenteritis were observed.