Publications by authors named "K V Kuleshov"
Article Synopsis
- The case report describes a patient with chronic lymphocytic B cell leukemia (B-CLL) who developed skin lesions that were initially misdiagnosed as leukemia cutis but were later identified as cutaneous borreliosis (specifically lymphocytoma).
- Borrelia bacteria were successfully isolated from the patient’s skin lesions, and antibiotic treatment resulted in significant improvement, although a second course of antibiotics was necessary for full resolution.
- After 11 years of follow-up, the patient remained free of skin lesions and stable in her B-CLL condition without needing chemotherapy, highlighting the interplay between Lyme disease and pre-existing malignancies.
is an emerging tick-borne human pathogen in the Northern hemisphere. The aim of the current study was to compare whole genome sequences of isolates from different continents. Using a combination of Illumina and PacBio platforms and a novel genome assembly and plasmid typing pipeline, we reveal that the 21 sequenced isolates and publically available genomes from North America, Asia, and Europe form genetically distinct populations and cluster according to their geographical origin, where distinct species are endemic.
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- - The Borrelia burgdorferi species complex causes Lyme borreliosis, but understanding the genetic differences among its various genospecies is hindered by challenges in assembling complete genomes due to their complex structures and numerous plasmids.
- - Previous genome assembly techniques have proven inadequate, but researchers used advanced HiFi PacBio sequencing and a refined workflow to successfully achieve gap-free and high-quality genome assemblies for Borrelia, including both chromosomal and plasmid sequences.
- - The study concludes that utilizing high-fidelity sequencing and an optimized reconstruction pipeline can effectively resolve complex microbial genomes, potentially benefiting future genomic research on other microorganisms.
is an important foodborne pathogen worldwide. Ciprofloxacin and extended-spectrum cephalosporins are the common first-line antimicrobial drugs for the treatment of salmonellosis, antimicrobial resistance genes for which are mostly transferred via plasmids. The goal of this work was to perform genomic analysis of plasmids from foodborne isolates obtained in Russia based on whole-genome sequencing.
View Article and Find Full Text PDFPolymyxin resistance, determined by genes located on plasmid DNA, currently poses a high epidemiological threat. Non-typhoid (NTS) are one of the key pathogens causing diarrheal diseases. Here, we report the isolation and whole genome sequencing of multidrug colistin-resistant/susceptible isolates of non-typhoid serovars carrying genes.
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