ATG8 delipidation is not universally critical for autophagy in plants.

Intracellular recycling via autophagy is governed by post-translational modifications of the autophagy-related (ATG) proteins. One notable example is ATG4-dependent delipidation of ATG8, a process that plays critical but distinct roles in autophagosome formation in yeast and mammals. Here, we aim to elucidate the specific contribution of this process to autophagosome formation in species representative of evolutionarily distant green plant lineages: unicellular green alga Chlamydomonas reinhardtii, with a relatively simple set of ATG genes, and a vascular plant Arabidopsis thaliana, harboring expanded ATG gene families.

A pilot study of the WE BEAT Well-Being Education Programme to build resilience in adolescents with heart disease.

Objective(s): To examine feasibility, acceptability, and preliminary effectiveness of a novel group-based telemedicine psychoeducation programme aimed at supporting psychological well-being among adolescents with Fontan-palliated CHD.

Study Design: A 5-week telemedicine psychoeducation group-based programme (WE BEAT) was developed for adolescents ( = 20; 13-18 years) with Fontan-palliated CHD aimed at improving resiliency and psychological well-being. Outcome measures included surveys of resilience (Connor-Davidson Resilience Scale), benefit finding (Benefit/Burden Scale for Children), depression, anxiety, peer relationships, and life satisfaction (National Institutes of Health Patient-Reported Outcomes Measurement Information System scales).

Provider Perceptions of Outcomes in the Pediatric Cardiac Intensive Care Unit.

In the pediatric cardiac intensive care unit (PCICU), predicting the complications and long-term impact of extracorporeal membrane oxygenation (ECMO) and cardiopulmonary resuscitation (CPR) is influenced by the providers' perceptions. Little is understood about such perceptions as they relate to provider role and experience. A multi-disciplinary group in the PCICU of a single center was surveyed regarding two patient scenarios: (1) ECMO after cardiac surgery, and (2) cardiac arrest with need for CPR.

Elevating diversity, inclusion, and health equity in Pediatric Heart Network Scholars grant funding: unique opportunities and lessons learned.

There is a growing awareness that diversity, health equity, and inclusion play a significant role in improving patient outcomes and advancing knowledge. The Pediatric Heart Network launched an initiative to incorporate diversity, health equity, and inclusion into its 2021 Scholar Award Funding Opportunity Announcement. This manuscript describes the process of incorporating diversity, health equity, and inclusion into the Pediatric Heart Network Scholar Award and the lessons learned.

The vacuolar K/H exchangers and calmodulin-like CML18 constitute a pH-sensing module that regulates K status in Arabidopsis.

Shifts in cytosolic pH have been recognized as key signaling events and mounting evidence supports the interdependence between H and Ca signaling in eukaryotic cells. Among the cellular pH-stats, K/H exchange at various membranes is paramount in plant cells. Vacuolar K/H exchangers of the NHX (Na,K/H exchanger) family control luminal pH and, together with K and H transporters at the plasma membrane, have been suggested to also regulate cytoplasmic pH.