Publications by authors named "K Toyooka"

Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is an immune-mediated disease that mainly affects the peripheral nerves and nerve roots and typically presents with distal dominant motor and sensory disturbances as clinical symptoms. Central nervous system (CNS) demyelination with inflammation occurs infrequently in patients with CIDP. Here, we present a unique autopsy report of CIDP causing severe demyelination along the entire spinal cord.

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Live imaging of secretory cargoes is a powerful method for understanding the mechanisms of membrane trafficking. Inducing the synchronous release of cargoes from an organelle is key for enhancing microscopic observation. We developed an optical cargo-releasing method, 'retention using dark state of LOV2' (RudLOV), which enables precise spatial, temporal, and quantity control during cargo release.

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Article Synopsis
  • - The study investigates how SARS-CoV-2 buds and transports its virions from the ERGIC to the cell surface, revealing that the vesicles involved have a protein coat known as coatomer complex I (COPI).
  • - Researchers observed that during infection, the distribution of COPI and the ERGIC changed, suggesting they play a role in the virus's replication process.
  • - Depleting a key COPI component, COPB2, confined the SARS-CoV-2 virions within the ERGIC and significantly reduced viral release, indicating that targeting COPI could be a potential strategy for developing antiviral treatments.
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Chloroplasts are photosynthetic organelles that evolved through the endosymbiosis between cyanobacteria-like symbionts and hosts. Many studies have attempted to isolate intact chloroplasts to analyze their morphological characteristics and photosynthetic activity. Although several studies introduced isolated chloroplasts into the cells of different species, their photosynthetic activities have not been confirmed.

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Land plants have evolved a hydrophobic cuticle on the surface of aerial organs as an adaptation to ensure survival in terrestrial environments. Cuticle is mainly composed of lipids, namely cutin and intracuticular wax, with epicuticular wax deposited on plant surface. The composition and permeability of cuticle have a large influence on its ability to protect plants against drought stress.

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