Effect of endodontic irrigants on biofilm matrix polysaccharides.

Aim: To specifically investigate the effect of endodontic irrigants at their clinical concentration on matrix polysaccharides of cultured biofilms.

Methodology: Saccharolytic effects of 3% H O , 2% chlorhexidine (CHX), 17% EDTA, 5% NaOCl and 0.9% saline (control) were tested using agarose (α 1-3 and β 1-4 glycosidic bonds) blocks (n = 3) in a weight assay.

Available chlorine consumption from NaOCl solutions passively placed in instrumented human root canals.

Aim: To monitor chlorine consumption from nonagitated aqueous sodium hypochlorite (NaOCl) solutions in human root canals using a recently developed assay, which can determine the order of magnitude of available chlorine in small volumes of liquid.

Methodology: The root canals of 80 extracted single-rooted human teeth were instrumented to ProTaper Universal F4 and irrigated using 1% NaOCl. Subsequently, canals were irrigated with copious amounts of deionized water to rinse out the residual chlorine.

A new method to assess available chlorine in small volumes of liquid.

Introduction: There is no robust and simple way to quantify available chlorine from small volumes such as human root canals. Therefore, a new method was developed and assessed.

Methods: Standardized size-40 paper points were soaked in a 15% (w/v) potassium iodide solution for 1 minute.