Characterization of the high-affinity oligosaccharide-binding site of the 205-kDa porcine large granular lymphocyte lectin, a member of the leukocyte common antigen family.

Membrane lectins of mammalian large granular lymphocytes are thought to be important receptors in their non-major-histocompatibility complex-restricted activation. A triantennary desialylated oligosaccharide has been reported as the most effective triggering structure [Pospísil M., Kubrycht J.

Peripheral membrane molecules of leukocytes and NK cytotoxicity.

Some leukocyte effector cell-surface molecules movement toward the adjoining target cells takes place during the reaction of NK cytotoxicity (NK R). The majority of the moving molecules are usually anchored via a divalent-ion-dependent interaction (PMM-M2+). The released PMM-M2+ can interact also with the secreted tumor necrosis factor alfa (TNF-alpha).

[Analysis of the polypeptide profile in insulin preparations using HPLC].

The purity of insulin preparations which is important during clinical administration of this hormone can be assessed in several ways. The authors suggested and tested a method for analysis of the polypeptide composition of insulin preparations which makes quantitative and qualitative evaluation of the insulin and non-insulin character possible, as well as their separation according to the shape and size of biological macromolecules by gel filtration on HPLC equipment. The described procedure makes possible a more detailed analysis of insulin preparations when the method is used in combination with electromigration and immunological estimations.

Cloning of a thermostable alpha-amylase gene from Thermomonospora curvata and its expression in Streptomyces lividans.

The gene from Thermomonospora curvata CCM 3312 coding for thermostable alpha-amylase (tam) has been cloned in Streptomyces lividans TK 24 and localized to a 2.6 kb HindIII-BamHI fragment of DNA. The data presented here show that the tam gene is expressed at a high level in S.

Production of quinomycin A in Streptomyces lasaliensis.

In addition to lasalocid, an oligoether coccidiostatic compound, other compounds are synthesized by Streptomyces lasaliensis. Mutants producing either of two antibiotics, lasalocid A or quinomycin A (an antibiotic of quinoxaline character), were obtained by natural selection and by mutagenesis. Methods of isolation, purification and estimation of both compounds were established.