Optimization of process parameters for the production of collagen peptides from fish skin ( using response surface methodology and its characterization.

The study optimized the hydrolysis conditions for the production of fish collagen peptides from skin of Malabar grouper () using response surface methodology. The hydrolysis was done with enzymes pepsin, papain and protease from bovine pancreas. Effects of process parameters viz: pH, temperature, enzyme substrate ratio and hydrolysis time of the three different enzymes on degree of hydrolysis were investigated.