Customized Design of Host-Independent T7 Expression System (HITES) for a Broad Host Range.

Efficient methods and universal DNA elements are eagerly required for the expression of proteins and the production of target chemicals in synthetic biology and metabolic engineering. This paper develops a customized-design approach by utilizing the host-independent T7 expression system (HITES), which facilitates the rational design and rapid construction of T7 expression systems. Firstly, the EL (Upper-limit value of initial enzyme activity) value is discovered to play a pivotal factor in the successful construction of the T7 expression system, different host strains exhibit varying EL values, and this study presents a method to measure the EL values.

Targeting N-Myc in neuroblastoma with selective Aurora kinase A degraders.

The N-Myc transcription factor, encoded by MYCN, is a mechanistically validated, yet challenging, target for neuroblastoma (NB) therapy development. In normal neuronal progenitors, N-Myc undergoes rapid degradation, while, in MYCN-amplified NB cells, Aurora kinase A (Aurora-A) binds to and stabilizes N-Myc, resulting in elevated protein levels. Here, we demonstrate that targeted protein degradation of Aurora-A decreases N-Myc levels.

Transcriptionally downregulated GABAergic genes associated with synaptic density network dysfunction in temporal lobe epilepsy.

Purpose: Temporal lobe epilepsy (TLE) is a brain network disorder closely associated with synaptic loss and has a genetic basis. However, the in vivo whole-brain synaptic changes at the network-level and the underlying gene expression patterns in patients with TLE remain unclear.

Methods: In this study, we utilized a positron emission tomography with the synaptic vesicle glycoprotein 2 A radioligand [F]SynVesT-1 cohort and two independent transcriptome datasets to investigate the topological properties of the synaptic density similarity network (SDSN) in TLE and its correlation with significantly dysregulated risk genes.

Improving future agricultural sustainability by optimizing crop distributions in China.

Improving agricultural sustainability is a global challenge, particularly for China's high-input and low-efficiency cropping systems with environmental tradeoffs. Although national strategies have been implemented to achieve Sustainable Development Goals in agriculture, the potential contributions of crop switching as a promising solution under varying future climate change are still under-explored. Here, we optimize cropping patterns spatially with the targets of enhancing agriculture production, reducing environmental burdens, and achieving sustainable fertilization across different climate scenarios.

Research on Wireless Passive Ultrasonic Thickness Measurement Technology Based on Pulse Compression Method.

Fixed-point thickness measurement is commonly used in corrosion detection within petrochemical enterprises, but it suffers from low detection efficiency for localized thinning, limitations regarding measurement locations, and high equipment costs due to insulation and cooling layers. To address these challenges, this paper introduces a wireless passive ultrasonic thickness measurement technique based on a pulse compression algorithm. The research methodology encompassed the development of mathematical and circuit models for single coil and wireless energy transmission, the proposal of a three-terminal wireless energy mutual coupling system, and the establishment of a finite element model simulating the ultrasonic body wave thickness measurement and wireless energy transmission system.