Effect of tolfenamic acid on the metabolism of the main connective tissue components in rats.

Tolfenamic acid (Clotam) has been used in the therapy of rheumatic diseases for some years. Regarding its chemical structure it belongs to the group of fenamates. The effect of tolfenamic acid on the synthesis of collagen and proteoglycans in granulation tissue, skin or cartilage of rat weanlings was tested and compared with the action of mefenamic acid.

Protein binding of tolfenamic acid in the plasma from patients with renal and hepatic disease.

The protein binding of tolfenamic acid in plasma from patients with renal and hepatic disorders was studied by equilibrium dialysis. Drug binding to the cellular components of whole blood and blood cell suspensions was also measured. Salicylic acid was used as the reference drug in all experiments.

Comparison of saliva and urine samples in thin-layer chromatographic detection of central nervous stimulants.

The excretion of amphepramone, amphetamine, ephedrine and prolintane was estimated qualitatively from saliva and urine using thin-layer chromatography (TLC) 1, 2, 4, 8, 12 and 24 h after a single intake of therapeutic doses by healthy volunteers. The purpose was to compare the suitability of these two biological fluids for doping tests and it was therefore important that either the drug or its metabolites should be found in all subjects. Ephedrine was the only one to be detected regularly in saliva already 1 h after drug intake, and it was found there up to 8 h, while in urine it was found up to 24 h.