The floor plate of the hindbrain is a highly specialized gland. Immunocytochemical and ultrastructural characteristics.

Although the participation of the floor plate (FP) in the differentiation of neurons in the ventral spinal cord is well established, the function of the FP at the level of the hindbrain is not known. The present investigation was focused on the FP of the rat hindbrain. We used an antibody specifically labeling the rat FP (FP4) and an antiserum raised against the secretory glycoproteins of the subcommissural organ that also reacts with an intracellular material in the FP.

Light- and electron-microscopic immunocytochemical investigation of the subcommissural organ using a set of monoclonal antibodies against the bovine Reissner's fiber.

Ten monoclonal antibodies (Mabs) against glycoproteins of the bovine Reissner's fiber (RF) have been used in a structural and ultrastructural immunocytochemical investigation of the bovine subcommissural organ (SCO) and RF. The SCO of other vertebrate species has also been studied. For comparison, polyclonal antibodies against bovine RF (AFRU) were used.

Light- and electron-microscopic investigation of the rat subcommissural organ grafted under the kidney capsule, with particular reference to immunocytochemistry and lectin histochemistry.

There is increasing evidence that, in the rat, a serotonin-mediated neural input may have an inhibitory influence on the secretory activity of the subcommissural organ (SCO). In the present investigation the rat SCO was studied 7, 30 and 90 days after transplantation under the kidney capsule, an area devoid of local serotonin-containing nerves. The grafted tissue was examined by use of immunocytochemistry employing a series of primary antisera, lectin histochemistry and transmission electron microscopy.

Effects of colchicine on the hypothalamo-neurohypophysial system of chronically salt-loaded rats.

The effect of colchicine on the hypothalamo-neurohypophysial system of rats drinking 2% NaCl for 4, 6, or 90 days was investigated. Colchicine was injected at time 0 for the 4-day group and 48 h before sacrifice for the 6- and 90-day groups. Each experimental group was divided into two subgroups that were injected with 7 micrograms colchicine into either the cisterna magna or the lateral cerebral ventricle.

Ontogenetical development of the chick and duck subcommissural organ. An immunocytochemical study.

The ontogenetical development of the subcommissural organ (SCO) was investigated in chick embryos collected daily from the 1st to the 21st day in incubation. Some duck embryos, and adult chickens and ducks were also studied. Immunocytochemistry using an anti-Reissner's fiber (RF) serum as the primary antibody was the principal method used.