The effect of arachidonic acid on the M current of NG108-15 neuroblastoma x glioma hybrid cells.

The M current, IM, a voltage-dependent non-inactivating K+ current, was recorded in NG108-15 neuroblastoma x glioma hybrid cells, using the whole-cell mode of the patch-clamp technique. We studied the effect of arachidonic acid, other fatty acids and inhibitors of the arachidonic acid metabolism. In relatively high concentrations (25-50 microM) arachidonic acid first increased and later decreased the current, Ih, which holds the membrane potential at -30 mV and mainly flows through open M channels.