Publications by authors named "K Saga"
Evaluation of the digestion protocol of mouse neonatal epidermis for single-cell RNA sequencing.
Biochem Biophys Res Commun
January 2025
The skin is primarily composed of keratinocytes and forms an effective barrier between the organism and external environment. Neonatal skin analysis is essential for understanding developmental processes and rare skin diseases. However, efficient single-cell dissociation methods for the neonatal mouse epidermis remain underexplored.
View Article and Find Full Text PDFThe tumor-infiltrating lymphocyte (TIL) is a crucial factor in controlling tumor growth. A therapeutic method activating TIL is desired for treating patients with metastatic tumors. Here, we show that treating a local tumor with a combination therapy of UV-irradiated hemagglutinating virus of Japan envelope (HVJ-E) plus agonist antibodies, including OX40, against T cell costimulatory molecules induces systemic anti-tumor effects in a T cell-dependent manner in multiple cancer cell lines.
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- Mouse orthotopic liver transplantation is a valuable method for studying liver ischemia and reperfusion injury, but it has technical challenges that hinder its use in research.
- The main difficulty lies in vascular reconstruction, especially for the portal vein and inferior vena cava, which can be improved by using plastic cuffs instead of sutures.
- This text aims to offer a technical guide on the cuff technique for vascular reconstruction, helping microsurgeons enhance their skills and advance liver research.
Cilia are organelles involved in motility and sensory transduction, but how these two functions coexist has not been elucidated in depth. Here, the involvement of the ciliary transient receptor potential (TRP) channel TRP11 in mechanoresponses is studied in using a TRP11-knockout mutant. The mutant has defects in the conversion of the bending mode of the cilium from forward to reverse when tapped with a glass rod, the detachment of cilia when shear is applied, the increase in ciliary beat frequency upon application of mechanical agitation by vortex mixing, and the initiation of gliding while both cilia are attached in opposite directions to a glass surface.
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- HIF-PHI, specifically roxadustat, is a new treatment for renal anemia that works by boosting erythropoietin levels but has raised concerns about potential side effects, particularly hypothyroidism.
- A study found a significant link between roxadustat use and hypothyroidism in real-world scenarios, while another HIF-PHI, daprodustat, did not show these signals.
- Patients using roxadustat should be monitored for thyroid function changes, as around 50% of hypothyroidism cases appeared within the first 50 days of treatment, regardless of the patient's age or sex.