Publications by authors named "K S Asrud"
Article Synopsis
- The development of organoid models, especially for the liver, addresses the limitations of traditional 2D cell culture by creating more physiologically relevant systems that better mimic native tissue.
- The new approach eliminates the need for 2D patterning and extracellular matrices, using small molecules to replicate embryonic liver development, resulting in liver-like organoids with complex cellular structures.
- These liver organoids demonstrate critical functions such as drug metabolism and protein production, and can be transplanted into mice, maintaining their functionality and offering potential for applications in therapy, drug testing, and disease modeling.
Previous studies demonstrate essential roles for the exchange proteins directly activated by cAMP 1 and 2 (Epac1 and Epac2; here collectively referred to as Epac) in the brain. In the hippocampus, Epac contributes to the control of neuronal growth and differentiation and has been implicated in memory and learning as well as in anxiety and depression. In the present study we address the hypothesis that Epac affects hippocampal cellular responses to acute restraint stress.
View Article and Find Full Text PDFEpac1 (Exchange protein directly activated by cAMP 1) limits fluid loss from the circulation by tightening the endothelial barrier. We show here that Epac1 mice, but not Epac2 mice, have prolonged bleeding time, suggesting that Epac1 may limit fluid loss also by restraining bleeding. The Epac1 mice had deficient in vitro secondary hemostasis.
View Article and Find Full Text PDFBackground: Obesity is still considered a risk factor for cardiovascular disease, although more recent knowledge also suggests obesity to be associated with reduced morbidity and mortality - the "obesity paradox". This study explores if long-term feeding of an obesogenic high fat diet renders the myocardium less susceptible to ischemic-reperfusion induced injury via Epac-dependent signaling.
Methods: Wild type (wt), Epac1 (Epac1) and Epac2 (Epac2) deficient mice were fed a high fat (HFD) or normal chow diet (ND) for 33 ± 1 weeks.